文献类型：期刊文献

英文题名：Phylogenetic analysis of newly isolated grass carp reovirus

作者：Yan, Xiu-ying[1];Wang, Ya[1];Xiong, Ling-fang[1];Jian, Ji-chang[1];Wu, Zao-he[2]

机构：[1]Guangdong Ocean Univ, Guangdong Key Lab Pathogen Biol & Epidemiol Aquat, Zhanjiang 524088, Peoples R China;[2]Zhongkai Univ Agr & Engn, Guangzhou 510225, Guangdong, Peoples R China

年份：2014

卷号：3

期号：1

外文期刊名：SPRINGERPLUS

收录：SCI-EXPANDED(收录号：WOS:000359023800002)、、Scopus(收录号：2-s2.0-84899539144)、WOS

基金：The study was supported by the National 973 Plan Project in China (No. 2009CB118704). The authors would like to thank Dr. JY Shen for her help.

语种：英文

外文关键词：Grass carp reovirus (GCRV); Identification; Phylogenetic relationship; Genotype

外文摘要：Grass carp reovirus (GCRV) is a causative agent of haemorrhagic disease in grass carp that drastically affects grass carp aquaculture. Here we report a novel GCRV isolate isolated from sick grass carp that induces obvious cytopathic effect in CIK cells and name it as GCRV096. A large number of GCRV 096 viral particles were found in the infected CIK cells by electron microscope. The shape, size and the arrangement of this virus were similar to those of grass carp reovirus. With the primers designed according to GCRV 873 genome sequences, specific bands were amplified from sick grass carp and the infected CIK cells. The homology rates among vp4, vp6 and vp7 gene in GCRV 096 and those of some GCRV isolates were over 89%. In this study, the sequences of vp4, vp6 and vp7 were used to analyse sequence variation, phylogenetic relationships and genotypes in twenty five GCRV isolates. The results indicated these twenty five GCRV isolates should be attributed to four genotypes. And there were no obvious characteristics in the geographical distribution of GCRV genotype. The study should provide the exact foundation for developing more effective prevention strategies of grass carp haemorrhagic disease.