文献类型：期刊文献

英文题名：A novel bacteriocin PE-ZYB1 produced by Pediococcus pentosaceus zy-B isolated from intestine of Mimachlamys nobilis: Purification, identification and its anti-listerial action

作者：Zhang, Ying[1]; Yang, Jingming[1]; Liu, Ying[1]; Wu, Yaqian[1]; Fang, Zhijia[1]; Wang, Yaling[1]; Sun, Lijun[1]; Deng, Qi[1]; Gooneratne, Ravi[2]; Xiao, Lixia[3]

机构：[1] College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, Zhanjiang, 524088, China; [2] Department of Wine, Food and Molecular Biosciences, Lincoln University, Lincoln 7647, Canterbury, New Zealand; [3] School of Food Science and Engineering, Yangzhou University, Yangzhou, 225127, China

年份：2020

卷号：118

外文期刊名：LWT

收录：EI(收录号：20194507626209)、Scopus(收录号：2-s2.0-85074326297)

语种：英文

外文关键词：Listeria - High performance liquid chromatography - Column chromatography - Diseases - Heat resistance - Cytology - Ion exchange

外文摘要：A novel bacteriocin, PE-ZYB1, was produced by Pediococcus pentosaceus zy-B that was isolated from the intestine of Mimachlamys nobilis and collected from the South China Sea. The bacteriocin PE-ZYB1 was purified using acetic ether extraction, Sephadex LH-20 column chromatography, sepharose cation exchange chromatography and reversed-phase high-performance liquid chromatography (RP-HPLC), in succession. Its molecular mass was determined to be MW 2019.22 Da, and its N-terminal amino acid sequence was DLFNKFNRSMQYGNGVE. The PE-ZYB1 was confirmed as a novel bacteriocin through a homology search, using UniProt BLAST (NO. A0A1Y0VLN8). Bacteriocin PE-ZYB1 was found to have extensive spectrum antimicrobial activity against both Gram-positive and Gram-negative bacteria. Bacteriocin PE-ZYB1 showed resistance to heat, was highly stable over pH 2–7 and was sensitive to trypsin. Increased extracellular electrical conductivity was detected for Listeria. monocytogenes treated by bacteriocin PE-ZYB1. Furthermore, bacteriocin PE-ZYB1 bactericidally destroyed the cell membrane integrity of L. monocytogenes, resulting in cell membrane perforation and permeabilization. These results indicate the potential of the bacteriocin PE-ZYB1 for use as a food bio-preservative. ? 2019 Elsevier Ltd