文献类型：期刊文献

英文题名：Pancreatic β-cell replacement: advances in protocols used for differentiation of pancreatic progenitors to β-like cells

作者：Ghani, Muhammad Waseem[1];Ye, Li[1];Yi, Zhao[1];Ghani, Hammad[2];Birmani, Muhammad Waseem[1];Nawab, Aamir[1];Cun, Lang Guan[1];Bin, Liu[1];Mei, Xiao[1]

机构：[1]Guangdong Ocean Univ, Agr Coll, Dept Livestock Prod & Management, Zhanjiang 524088, Guangdong, Peoples R China;[2]Univ Gujrat, Nawaz Sharif Med Coll, Punjab, Pakistan

年份：2019

卷号：57

期号：3

起止页码：101

外文期刊名：FOLIA HISTOCHEMICA ET CYTOBIOLOGICA

收录：SCI-EXPANDED(收录号：WOS:000489034200001)、、Scopus(收录号：2-s2.0-85072945298)、WOS

语种：英文

外文关键词：beta-cell replacement; transdifferentiation; pancreatic duct cells; acinar cells; centroacinar cells; endocrine cells; mesenchymal stem cells; beta-like cells

外文摘要：Insulin-producing cells derived from in vitro differentiation of stem cells and non-stem cells by using different factors can spare the need for genetic manipulation and provide a cure for diabetes. In this context, pancreatic progenitors differentiating to beta-like cells garner increasing attention as beta-cell replacement source. This kind of cell therapy has the potential to cure diabetes, but is still on its way of being clinically useful. The primary restriction for in vitro production of mature and functional beta-cells is developing a physiologically relevant in vitro culture system which can mimic in vivo pathways of islet development. In order to achieve this target, different approaches have been attempted for the differentiation of pancreatic stem/progenitor cells to beta-like cells. Here, we will review some of the state-of-the-art protocols for the differentiation of pancreatic progenitors and differentiated pancreatic cells into beta-like cells with a focus on pancreatic duct cells.