文献类型：期刊文献

中文题名：卵形鲳鲹促性腺激素β亚基的克隆鉴定及其受雌二醇的调控

英文题名：Cloning and characterization of gonadotropinβsubunit genes in pompano(Trachinotus ovatus)and their regulation by estradiol

作者：黄春艳[1];陈华谱[1];郭煜文[1];王岩[1];杨浩[1];李广丽[1]

机构：[1]广东省名特优鱼类生殖调控与繁育工程技术研究中心/广东省水产经济动物病原生物学及流行病学重点实验室/广东省海水养殖生物育种工程实验室/广东海洋大学水产学院,广东湛江524088

年份：2025

卷号：64

期号：1

起止页码：172

中文期刊名：中山大学学报（自然科学版）（中英文）

外文期刊名：Acta Scientiarum Naturalium Universitatis Sunyatseni

收录：北大核心2023、CSTPCD、、北大核心

基金：广东省重点领域研发计划项目(2021B0202020002);湛江市科技计划项目(2022A01214);阳江市科技计划项目(2022011,SDZX2023027,SDZX2021041);广东省现代化海洋牧场适养品种核心技术攻关项目(2024-MRB-00-001)。

语种：中文

中文关键词：卵形鲳鲹(Trachinotus ovatus);促性腺激素(GtH);雌激素受体(ER);17β-雌二醇(E2)

外文关键词：pompano(Trachinotus ovatus);gonadotropin hormones(GtH);estrogen receptor(ER);17β-estradiol(E2)

中文摘要：促性腺激素(GtH,gonadotropin hormone)是垂体合成和分泌的一类重要糖蛋白激素,包括促卵泡生成素(Fsh,follicle stimulating hormone)和促黄体生成素(Lh,luteinizing hormone),在促进性腺发育和成熟过程中发挥重要作用。本研究成功克隆了卵形鲳鲹(Trachinotus ovatus)fshβ和lhβ基因的开放阅读框(ORF,open reading frame)序列,fshβORF长度为363 bp,lhβORF长度为447 bp。通过荧光定量PCR技术(qPCR,quantitative realtime PCR)检测,发现fshβ和lhβ基因在卵形鲳鲹的下丘脑-垂体-性腺轴(HPG,hypothalamus-pituitary-gonadal axis)显著表达,在垂体中的表达水平最高,其次是下丘脑和性腺。此外,通过体外实验评估17β-雌二醇(E2,17β-estradiol)对卵形鲳鲹垂体组织中fshβ和lhβ基因表达的影响。结果显示,10μmol/L E2处理6 h后,GtHβ亚基基因的表达被极显著抑制。最后,使用雌激素受体(ER,estrogen receptor)拮抗剂体外孵育卵形鲳鲹垂体组织,发现广谱性拮抗剂Fulvestrant、ERβ拮抗剂Cyclofenil和ERα拮抗剂MPP(MPP dihydrochloride)均能够消除E2对GtHβ亚基基因表达的抑制作用。综上所述,研究结果表明E2对卵形鲳鲹GtH的分泌具有负反馈调节作用,为卵形鲳鲹生殖调控机制提供了有价值的见解。

外文摘要：Gonadotropin hormones(GtH)are a crucial class of glycoprotein hormones synthesized and secreted by the pituitary,playing a pivotal role in promoting gonadal development and maturation.In this study,we successfully cloned the open reading frame(ORF)sequences of fshβand lhβfrom pompano(Trachinotus ovatus),with fshβORF length of 363 bp and lhβORF length of 447 bp.Using quantitative real-time PCR(qPCR),we observed expression of fshβand lhβin various tissues of pompano,with the highest levels detected in the pituitary followed by the hypothalamus.Furthermore,in vitro experiments were conducted to assess the effect of 17β-estradiol(E2)on fshβand lhβexpression in pompano pituitary.Results showed a significant inhibition of fshβand lhβexpression after 6 h of treatment with 10μmol/L E2.Lastly,using estrogen receptor(ER)antagonists for in vitro incubation of pompano pituitary tissue,we demonstrated that broad-spectrum antagonist Fulvestrant,ERβantagonist Cyclofenil,and ERαantagonist MPP dihydrochloride could alleviate the inhibitory effect of E2on fshβand lhβexpression.Overall,our findings suggest a negative feedback regulation of E2 on GtH secretion in pompano,providing valuable insights into the reproductive regulation mechanism of pompano.