文献类型：期刊文献

中文题名：狗舌草提取物的长期和蓄积毒性及特殊毒性研究

英文题名：Studies on chronic toxicity,accumulation and special toxicities of extract from Tephroseris kirilowii (Turcz.) Holub

作者：聂芳红[1];陈进军[2];王建华[3];史志诚[4]

机构：[1]广东海洋大学食品科技学院,广东湛江524088;[2]广东海洋大学农业生物技术研究所,广东湛江524088;[3]西北农林科技大学动物医学院,陕西杨凌712100;[4]西北大学生态毒理研究所,陕西西安710069

年份：2008

卷号：36

期号：2

起止页码：45

中文期刊名：西北农林科技大学学报（自然科学版）

外文期刊名：Journal of Northwest A&F University(Natural Science Edition)

收录：CSTPCD、、北大核心2004、CSCD2011_2012、北大核心、CSCD

基金：广东省自然科学基金项目(05011789);广东省科技计划项目(2004B20201007)

语种：中文

中文关键词：狗舌草;乙醇提取物;长期毒性;蓄积毒性;特殊毒性;小鼠

外文关键词：Tephroseris kirilowii （Turcz.） Holub; ethanol extract; chronic toxicity; accumulation;

中文摘要：【目的】对狗舌草体积分数60%乙醇提取物的毒理学安全性进行评价。【方法】利用C57L-L小鼠,以生理盐水为阴性对照,环磷酰胺为阳性对照,进行狗舌草体积分数60%乙醇提取物的90 d长期毒性试验、30 d蓄积试验。并采用小鼠骨髓微核试验、小鼠生殖机能试验和小鼠胎儿外观畸形检查,对狗舌草体积分数60%乙醇提取物的长期毒性、蓄积毒性和特殊毒性进行评价。【结果】90 d长期毒性试验未见供试小鼠临床表现、体重和病理变化异常,30 d蓄积试验未见供试小鼠死亡,给药组与阴性对照组小鼠骨髓微核率无显著差异(P>0.05);与阴性对照组相比,用药后受孕鼠的受孕率、每窝活胎数、平均胎儿重、吸收胎儿数和死胎数均无明显变化(P>0.05),供试小鼠胎儿未见明显的外观畸形(P>0.05)。【结论】狗舌草体积分数60%乙醇提取物无长期毒性,无蓄积毒性,无致突变性和致畸胎性。

外文摘要：[Objective] The study is to assess the toxicological safety of 60% （V/V） alcoholic extract from Tephroseris kirilowii Turcz Holub. [Method] With a negative control of normal saline and a positive control of cyclophosphamide, the chronic toxicity,accumulation and special toxicities were assessed by employing a range of C57L-L mice to conduct the tests of 90 day chronic toxicity,30-day accumulation,bonemarrow micronucleus and reproduction ability as well as fetal malformation of the T. kirilowii extract. [Result] There were no abnormalities in clinical exhibition, body weight and visible pathology in 90-day chronic toxicity test and no death in 30-day accumulation test. Being compared to the negative control group,the mouse bone-marrow micronucleus rate was not changed （P〉0.05） and there were no significant changes （P〉0.05） in numbers of live,absorbed and dead fetuses of the pregnant mice and no visible fetal malformation in the teratogenic test （P〉0.05）. [Conclusion] The T. kirilowii extract with 60% （V/V） ethanol did not cause chronic toxicity, accumulation,mutagenicity and teratogenicity.