文献类型：期刊文献

中文题名：湛江水产品中大肠埃希氏菌耐药性检测与分析

英文题名：Antimicrobial Susceptibility in Escherichia coli Isolated from Fishery Products in Zhanjiang

作者：丁秀琼[1];黄武[1];刘建芳[1];雷晓凌[2,3,4];麦嘉惠[2];黄健勇[2];聂芳红[2,3,4]

机构：[1]湛江海关技术中心,广东湛江524000;[2]广东海洋大学食品科技学院,广东湛江524088;[3]广东省水产品加工与安全重点实验室,广东湛江524088;[4]水产品深加工广东普通高等学校重点实验室,广东湛江524088

年份：2021

卷号：40

期号：4

起止页码：561

中文期刊名：水产科学

外文期刊名：Fisheries Science

收录：CSTPCD、、北大核心、CSCD、北大核心2020、CSCD_E2021_2022

基金：湛江市科技计划项目(2015A03022).

语种：中文

中文关键词：水产品;大肠埃希氏菌;耐药性;耐药基因

外文关键词：fishery product;Escherichia coli;drug resistance;drug resistance gene

中文摘要：采用K-B纸片扩散法,对从近江牡蛎、翡翠贻贝、菲律宾蛤仔、文蛤、华贵类栉孔扇贝等鲜活样品和罗非鱼片、熟虾等冷冻样品中分离的52株大肠埃希氏菌菌株进行青霉素类、喹诺酮类、单环内酰胺类和磺胺类等19种抗菌药物的药敏性分析,同时PCR扩增检测超广谱β-内酰胺酶(ESBLs)、质粒介导喹诺酮耐药(PMQR)基因,以检测湛江水产品中大肠埃希氏菌的耐药性及ESBLs、PMQR基因的携带情况。结果显示,52株大肠埃希氏菌菌株均对1种以上抗菌药物产生耐药性,其中对红霉素(90.4%)、复方新诺明(55.8%)、四环素(53.8%)、头孢噻肟(51.9%)的耐药率较高;76.9%的菌株表现出多重耐药,最多对9大类抗生素耐药;确证为产ESBLs菌株10株(19.2%),8株携带blaTEM基因,7株携带blaSHV基因,未检出blaCTX和blaOXA基因;检出PMQR菌株15株,检出率为28.8%,其中15株同时携带qnrA、aaac(6′)-Ib-c、qepA 3种耐药基因,9株携带qnrS基因,8株携带oqxB基因,1株携带qnrD基因,未检出qnrB、qnrC和oqxA耐药基因;2株大肠埃希氏菌同时携带ESBLs基因和PMQR基因。研究结果表明,湛江地区水产品中大肠埃希氏菌普遍耐药,且耐药谱广。菌株同时携带ESBLs基因和PMQR基因,有增加细菌耐药性的可能。

外文摘要：Antimicrobial susceptibility of 52 susceptible Escherichia coli strains isolated from fresh samples such as oyster Crassostrea ariakensis,green mussel Perna viridis,Manila clam Ruditapes philippinarum,hard clam Meretrix meretrix,and scallop Mimachlamys nobilis,and frozen samples of fish fillets and cooked shrimp to 19 antibacterial drugs including penicillins,quinolones,monocyclic lactams and sulfonamides by using K-B paper diffusion method,and then extended-spectrumβ-lactamase(ESBLs)and plasmid-mediated quinolone resistance(PMQR)genes were amplified by PCR to detect the resistance of E.coli in fishery products in Zhanjiang.Results showed that all of the 52 E.coli strains were resistant to more than one antibiotic,with higher resistance rate of erythromycin(90.4%),sulfamethoxazolethe(55.8%),trimethoprim(53.8%),and cefotaxime(51.9%)than that in other antibiotics.About 76.9%of E.coli showed multiple resistant of up to 9 categories of antibiotics,and 10 strains were confirmed to be ESBLs bacteria,with detection rate of 19.2%(10/52),8 strains carrying gene blaTEM,7 strains carrying gene blaSHV,without blaCTX and blaOXA genes.It was confirmed that 15 strains were PMQR bacteria,with detection rate of 28.8%(15/52),the all carrying qnrA,aac(6′)-Ib-c and qepA genes,9 strains carrying qnrS gene,8 strains carrying qnrD gene,and only 1 strain carrying oqxB gene,without qnrB,qnrC and oqxA genes.Both ESBLs gene and PMQR gene were carried by 2 E.coli isolates.The findings revealed that E.coli from fishery products in Zhanjian area was resistant to a wide range of antibiotics and that combination of ESBLs gene with PMQR gene enhanced the drug resistance of bacteria.