文献类型：期刊文献

英文题名：A sensitive colorimetric DNA biosensor for specific detection of the HBV gene based on silver-coated glass slide and G-quadruplex-hemin DNAzyme

作者：Li, Yubin[1];Liu, Sheng[1];Deng, Qiujuan[1];Ling, Liansheng[2]

机构：[1]Guangdong Ocean Univ, Coll Chem & Environm, Zhanjiang, Peoples R China;[2]Sun Yat Sen Univ, Sch Chem, Guangzhou, Guangdong, Peoples R China

年份：2018

卷号：90

期号：4

起止页码：699

外文期刊名：JOURNAL OF MEDICAL VIROLOGY

收录：SCI-EXPANDED(收录号：WOS:000424373300013)、、Scopus(收录号：2-s2.0-85038219761)、WOS

基金：Doctoral Initiating Project of Guangdong Ocean University Foundation for Natural Sciences (China), Grant number: R17013; The Natural Science Foundation of Guangdong Ocean University (China), Grant number: C16401

语种：英文

外文关键词：biochemical analysis; enzyme assays; hepatitis B virus; research and analysis methods; virus classification

外文摘要：A sensitive colorimetric DNA biosensor for specific detection of single stranded oligonucleotide (ssDNA) is proposed in this paper. The biosensor is based on silver-coated glass (SCGS) and G-quadruplex-hemin DNAzyme. Capture DNA is immobilized on the surface of SCGS by AgS bond. Signal DNA can be used to hybridize with the target DNA which is selected from the Hepatitis B virus(HBV) gene as target HBV DNA, and the HRP-mimicking G-quadruplex-hemin DNAzyme can be formed through the function of a guanine-rich fragment from signal DNA to catalyze the oxidation of 2,2-azinobis(3-ethylbenzothiozoline)-6-sulfonicacid (ABTS(2-)) by H2O2. The reaction will be monitored along the side of absorbance changes at 418nm and it can be viewed by naked eye with the change of color as well. Upon addition of target Hepatitis B virus(HBV) DNA, signal DNA could bind on the surface of SCGS, and the concentration of G-quadruplex-hemin DNAzyme immobilizing on the surface of SCGS is depended on that of target HBV DNA. Under the optimum conditions, the absorption was proportional to the concentration of target HBV DNA over the range from 0.5 to 100nM, with a detection limit of 0.2nM. In addition, the biosensor is target specific and practicability. This assay might open a new avenue for applying in the diagnosis of HBV disease in the future.