文献类型：期刊文献

中文题名：外源调环酸钙对盐碱胁迫下大豆幼苗生长及生理特性的影响

英文题名：Effects of exogenous prohexadione-calcium on growth and physiological characteristics of soybean seedlings under saline-alkali stress

作者：余明龙[1,2];黄露[1];郑殿峰[1,3];冯乃杰[1,3];牟保民[2];刘美玲[2]

机构：[1]广东海洋大学滨海农业学院,广东湛江524088;[2]黑龙江八一农垦大学农学院,黑龙江大庆163000;[3]广东海洋大学深圳研究院,广东深圳518108

年份：2022

卷号：41

期号：4

起止页码：683

中文期刊名：生态学杂志

外文期刊名：Chinese Journal of Ecology

收录：CSTPCD、、CSCD2021_2022、Scopus、北大核心、CSCD、北大核心2020

基金：国家自然科学基金项目(31871576);国家重点研发项目(2017YFD0201306-03);黑龙江省自然科学基金重点项目(ZD2017003)资助。

语种：中文

中文关键词：调环酸钙;大豆;盐碱胁迫;光合特性;AsA-GSH循环;

外文关键词：prohexadione-calcium;soybean;saline-alkali stress;photosynthetic characteristics;AsA-GSH cycle

中文摘要：为明确外源调环酸钙(Pro-Ca)缓解大豆幼苗盐碱胁迫的机理,以大豆‘合丰50’为试验材料,研究在110 mmol·L^(-1)复合盐碱胁迫下,叶面喷施100 mg·L^(-1)Pro-Ca对大豆幼苗生长、光合特性、抗氧化代谢、AsA-GSH循环以及渗透调节的影响。结果表明:盐碱胁迫显著抑制大豆幼苗生长,降低了净光合速率(P_(n))、叶绿素、可溶性蛋白、可溶性糖、蔗糖和淀粉含量;增加了过氧化氢酶(CAT)活性以及脯氨酸、活性氧(ROS)、丙二醛(MDA)和电解质渗漏率积累;与盐碱处理相比,喷施Pro-Ca能够改善大豆幼苗地上部和根系生长,提高叶绿素a、叶绿素b、类胡萝卜素和总叶绿素含量,维持较高的P_(n),促进蔗糖、果糖和淀粉的积累;显著上调叶片6种抗氧化酶(SOD、POD、CAT、GR、MDHAR和DHAR)活性、2种非酶抗氧化剂(AsA和GSH)水平和脯氨酸含量;而电解质渗漏率、O_(2)^(-)·产生速率以及MDA和H_(2)O_(2)含量显著降低。综上所述,调环酸钙通过调节抗氧化酶活性和AsA-GSH循环系统、增加渗透溶质积累、降低ROS损伤来提高大豆幼苗对盐碱胁迫的耐性。

外文摘要：This study was conducted to clarify the mechanism underlying the alleviation saline-alkali stress of exogenous prohexadione-calcium(Pro-Ca)of soybean seedlings.The soybean variety‘Hefeng 50’was used as experimental material to examine the effects of foliar spraying Pro-Ca of 100 mg·L^(-1)on the growth,photosynthetic characteristics,antioxidant metabolism,AsA-GSH cycle and osmotic adjustment of soybean seedlings under 110 mmol·L^(-1)saline-alkali stress.Results showed that saline-alkali stress significantly inhibited the growth of soybean seedlings,diminished the net photosynthetic rate(P_(n))and the contents of chlorophyll,soluble protein,soluble sugar,sucrose,and starch,and increased catalase(CAT)activity and the accumulation of proline,reactive oxygen species(ROS),malondialdehyde(MDA),and electrolyte leakage.Compared with the saline-alkali treatment,exogenous application of Pro-Ca improved aboveground and root growth of soybean seedlings,increased the contents of chlorophyll a,chlorophyll b,carotenoid,and total chlorophyll,maintain higher P_(n),and promote the accumulation of sucrose,fructose,and starch.Furthermore,the application of Pro-Ca drastically increased the activities of six antioxidant enzymes(SOD,POD,CAT,GR,MDHAR,and DHAR),the levels of two nonenzymatic antioxidants(AsA and GSH),and the content of proline in leaves.Meanwhile,the electrolyte leakage,O_(2) production rate,and contents of MDA and H_(2)O_(2) significantly decreased.In conclusion,the exogenous application of Pro-Ca can improve the tolerance of soybean seedlings to saline-alkali stress by regulating antioxidant enzymes and AsA-GSH cycle,increasing osmotic solute accumulation and reducing ROS damage.