文献类型：期刊文献

英文题名：Toxicity of chronic waterborne zinc exposure in the hepatopancreas of white shrimp Litopenaeus vannamei

作者：Liang, Zhi[1,2,3];Chen, Tianci[1,2,3];Yang, Furong[1,2,3];Li, Shuhong[1,2,3];Zhang, Shuang[1,4,5];Guo, Hui[1,2,3]

机构：[1]Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524025, Peoples R China;[2]Guangdong Higher Educ Inst, Guangdong Prov Key Lab Pathogen Biol & Epidemiol A, Zhanjiang 524025, Peoples R China;[3]Guangdong Higher Educ Inst, Key Lab Control Dis Aquat Econ Anim, Zhanjiang 524025, Peoples R China;[4]Minist Agr, Key Lab Aquat Livestock & Poultry Feed Sci & Techn, Zhanjiang 524025, Peoples R China;[5]Aquat Anim Precis Nutr & High Efficiency Feed Engn, Zhanjiang, Peoples R China

年份：2022

卷号：309

外文期刊名：CHEMOSPHERE

收录：SCI-EXPANDED(收录号：WOS:000868422500001)、、EI(收录号：20224112854457)、Scopus(收录号：2-s2.0-85139282955)、WOS

基金：Acknowledgments This research was supported by the National Natural Science Foun-dation of China (31600321) . We are especially grateful for the critical comments from the anonymous reviewers.

语种：英文

外文关键词：Zinc; Toxicity; Hepatopancreas; Transcriptome; Litopenaeus vannamei

外文摘要：Zinc (Zn) is necessary for the survival of aquatic organisms; nevertheless, the accumulation of Zn in excessive amounts may have toxic consequences. Few studies focusing on the biochemical, morphological, and tran-scriptional effects of aqueous Zn in Litopenaeus vannamei have been reported, and the underlying toxic mecha-nism remains largely unknown. The present study was performed to investigate the growth performance, morphological alterations, physiological changes, and transcriptional responses after Zn exposure at 0 (control), 0.01, 0.1, and 1 mg/L concentrations for 30 days in white shrimp L. vannamei hepatopancreas. The results found that survival rate (SR) and growth performance were significantly reduced in 1 mg/L Zn group. Significant structural damage and significant Zn accumulation in hepatopancreas were observed. The activities of trypsin and amylase (AMS), and the total antioxidant capacity (T-AOC) were attenuated, while the production of reactive oxygen species (ROS) and malondialdehyde (MDA) content were significantly increased after Zn exposure. Many differentially expressed genes (DEGs) were obtained after Zn exposure, and the majority of these DEGs were downregulated. Ten DEGs involved in oxidative stress, immunological response, apoptosis, and other processes were selected for qRT-PCR validation and the expression profiles of these DEGs kept well consistent with the transcriptome data, which confirmed the accuracy and reliability of the transcriptome results. Subsequently, we screened 12 genes to examine the changes of expression in different concentrations in more detail. All the results implying that Zn exposure caused severe histopathological changes and increased Zn accumulation in hepato-pancreas, altered immune, antioxidant and detoxifying response by regulating the gene expressions of related