文献类型：期刊文献

英文题名：Identification of 10 transcripts of FREP in penaeid shrimp Litopenaeus vannamei

作者：Hou, Fujun[1];Gao, Tiantian[1];Liu, Tao[1];Jia, Zhiying[1];Liu, Yongjie[1];Sun, Chengbo[2];Liu, Xiaolin[1]

机构：[1]Northwest A&F Univ, Coll Anim Sci & Technol, Shaanxi Key Lab Mol Biol Agr, Yangling 712100, Peoples R China;[2]Guangdong Ocean Univ, Coll Fisheries, Guangzhou 524088, Guangdong, Peoples R China

年份：2016

卷号：58

起止页码：436

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000387523100052)、、WOS

基金：This work was supported by the Agricultural Science and Technology Achievement Transformation Fund Project of Ministry of Science and Technology, China (no.2012GB2E200361), the Key Laboratory of Marine Biology, Institute of Oceanlogy, Chinese Academy of Sciences, Qianjiang distinguished experts of Hangzhou and Extension and Demonstration Program in Desalting Culture of Litopenaeus Vannamei New Breed "Ke Hai 1", Northwest A&F University, China (no.XNY2013-4) and Scientific planning and applying program, Shaanxi (2015KTTSNY01-01). Comments from anonymous reviewers have greatly improved the manuscript.

语种：英文

外文关键词：Fibrinogen-related proteins; Shrimp; Bacteria

外文摘要：Fibrinogen-related proteins (FREPs) are widely distributed in vertebrates and invertebrates and known having Fibrinogen-related domains (FReDs) which function in multiple aspects, especially in innate immune response as pattern recognition receptors. However, there is no any report about FREP in penaeid shrimp Litopenaeus vannamei. Here, totally 10 transcripts of FREP were isolated and named LvFREP1.1, 1.2 until 1.10. All of the 10 transcripts have high identity in their 3' ends and encode conserved FReDs. Since the 10 transcripts are highly similar we could not design any primers that can amplify a single transcript. We chose a pair of primers corresponding to part of LvFREP1.1 and LvFREP1.5 to examine their expression. Tissue distribution indicated LvFREP1.1 and LvFREP1.5 mRNA locates in hemocytes, gills, intestine, hearts and slightly in nerve. The expression of LvFREP1.1 and LvFREP1.5 behaves differently post bacteria and virus infection. Besides, recombinant LvFReD could agglutinate bacteria Vibrio harveyi in the presence of Ca2. These initial data presents the diversity of FREPs in penaeid shrimp and also push us to further explore their roles in shrimp immune response. (C) 2016 Elsevier Ltd. All rights reserved.