文献类型：期刊文献

中文题名：青石斑鱼微卫星标记的筛选及群体多态性分析

英文题名：Isolation and characterization of polymorphic microsateilite markers in Epinephelus awoara

作者：董秋芬[1];刘楚吾[1];郭昱嵩[1];刘丽[1];徐田军[1]

机构：[1]广东海洋大学水产学院,广东湛江524025

年份：2007

卷号：31

期号：6

起止页码：841

中文期刊名：水产学报

外文期刊名：Journal of Fisheries of China

收录：CSTPCD、、北大核心2004、Scopus、CSCD2011_2012、北大核心、CSCD

基金：广东省重大科技兴海项目(A200099A01);湛江市988科技攻关项目(2000-121-17)

语种：中文

中文关键词：青石斑鱼;基因组文库;PCR法;微卫星标记;遗传多样性

外文关键词：Epinephelus awoara;;genomic library;;PCR method;;microsatellite markers;;genetic diversity

中文摘要：微卫星DNA，又称短串联重复序列（STR）或简单序列重复（SSR），是广泛存在于真核生物基因组中的简单重复DNA片段，一般每个重复单位仅1～6个碱基，重复数为10～20次，其中动物体中以双核苷酸（CA／GT）n最为常见。根据微卫星DNA核心序列两端的保守序列设计引物扩增基因组DNA便可以找到相应的微卫星标记。微卫星标记已广泛应用于人类和动植物遗传育种、遗传图谱绘制、数量性状位点定位、标记辅助选择、遗传多样性评估等方面的研究。

外文摘要：Yellow grouper Epinephelus awoara is an economically important marine fish mainly distributed in northwest-Pacific, western central-Pacific and South China Sea, but the number of its population greatly decreased due to overfishing of grouper adults and juveniles, habitat damage and loss from bottom trawling, pollution, high demand for food and so on these years. E. awoara was listed in the 2005 IUCN Red List as a specie with DD （data deficient）. Since molecular markers are very useful tools to estimate germplasm resources, variety identification, genetic diversity analysis and preparation of genetic linkage map, the microsatellites of E. awoara were isolated and thus its micosatellite markers and genetic diversity were analysed. Firstly a library of partial small size fractionated genomic DNA was constructed with the E. awoara from South China Sea. 96 microsatellites in 28 recombinant positive clones were obtained through PCR screening the library with M13 universal primers and simple tandem repeats primer（CA） 15. Among these microsatellites, there were 39 perfect ones （ 40.6% ）, 30 imperfect ones （ 31.3% ）, 7 compound perfect ones（7.3% ） and 20 compound imperfect ones （20.8%）. The results indicated that microsatellite sequences characterized by （CA/GT） n were abundant in genomic DNA of E. awoara. 26 of 28 pairs of primers which were designed to analyze genomic DNA of E. awoara according to unique microsatellite flanking sequences with the software Primer 5. 0 could be amplified expected bands, of which 13 microsatellite markers were assessed genetic diversity for 19 individuals. As a consequence, the data showed that the average observed heterozygosity （Ho）, average expected heterozygosity （He ）, average polymorphism information content （PIC） and average Hardy-Weinberg departure value （D） of E. awoara were 0. 598 2, 0. 508 0, 0. 472 2 and 0. 150 3 respectively. The findings indicated that although the genetic diversity of E. awoara in South China Sea was rich, but it had been influenced by human behaviors to a certain extent.