文献类型：期刊文献

英文题名：Complete mitochondrial genome and assembled DNA barcoding analysis of Lutjanus fulgens (Valenciennes, 1830) and its comparison with other Lutjanus species

作者：Afriyie, Gyamfua[1];Wang, Zhongduo[1,2];Dong, Zhongdian[1];Ayisi Larbi, Christian[3];Asiedu, Berchie[4];Guo, Yusong[1]

机构：[1]Guangdong Ocean Univ, Coll Fisheries, Key Lab Aquaculture South China Sea Aquat Econ An, Zhanjiang 524025, Peoples R China;[2]Guangdong Ocean Univ, Coll Fisheries, Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang, Peoples R China;[3]Univ Dev Studies, Dept Fisheries & Aquat Resources Management, Tamale, Ghana;[4]Univ Energy & Nat Resources, Dept Fisheries & Aquaculture, Sunyani, Ghana

年份：2020

卷号：10

期号：15

起止页码：7971

外文期刊名：ECOLOGY AND EVOLUTION

收录：SCI-EXPANDED(收录号：WOS:000554952800001)、、Scopus(收录号：2-s2.0-85087758943)、WOS

基金：National Natural Science Foundation of China, Grant/Award Number: 31 and 972794; Project of Enhancing School with Innovation of Guangdong Ocean University, Grant/Award Number: 230419055

语种：英文

外文关键词：COIgene; complete mitogenome; DNA barcode; Ghana; management and conservation; phylogeny

外文摘要：Lutjanus fulgens(Valenciennes, 1830) is a teleost species classified under the family Lutjanidae which is a native of the Eastern Atlantic Ocean. Though highly commercialized due to its abundance and good taste, the production output has declined in recent years. This is an indication of the need for effective management and conservation measures. However, accurate species identification will ensure strategic management and conservation measure. DNA-based species identification has proven its reliability in this regard via precise species identification. Several researchers have confirmed the accuracy of DNAbarcode as a species identification tool as well as species phylogeny analysis based on both the complete mitogenome andCOIgene. Currently, nine specimens ofL. fulgenswere sampled from Ghana and subjected to DNA-based analysis, namely, complete mitochondrial DNAandCOIgene (DNA barcoding) analyses. The mitogenomic result revealed thatL. fulgensis made up of a 16,500 base pairs (bp) mtDNA which consists of 22 transfer RNAs, 13 protein-coding genes, and two ribosomal RNAs (GenBank Accession Number: MN398650). Furthermore, a sequence polymorphism analysis of theCOIgene (MN986442-MN986450) detected two haplotypes. These haplotypes were both collected from the same fish landing site which suggests a possible cryptic linage diversity in theL. fulgenspopulation at Vodza. According to the phylogeny examination, a close taxonomic relationship exists betweenL. fulgensandLutjanus buccanellacaused by a recent evolution termed as sympatric speciation. This study serves as a novel study for this species, building the foundation for future molecular-based study for this species and as a DNA barcode reference data.