文献类型：期刊文献

英文题名：Anti-inflammatory activity of flavonols via inhibiting MAPK and NF-ΚB signaling pathways in RAW264.7 macrophages

作者：Zhong, Ruting[1,2];Miao, Lingchao[1];Zhang, Haolin[1];Tan, Lihua[1,2];Zhao, Yuxin[1,2];Tu, Yanbei[1,2];Prieto, Miguel Angel[4];Simal-Gandara, Jesus[4];Chen, Lei[3];He, Chengwei[1,2];Cao, Hui[3,4]

机构：[1]Univ Macau, Inst Chinese Med Sci, Taipa, Macao, Peoples R China;[2]Univ Macau, Guangdong Hong Kong Macau Joint Lab Chinese Med &, Taipa, Macao, Peoples R China;[3]Guangdong Ocean Univ, Coll Food Sci & Technol, Guangdong Prov Key Lab Aquat Prod Proc & Safety, Zhanjiang 524088, Peoples R China;[4]Univ Vigo, Fac Sci, Dept Analyt & Food Chem, Nutr & Bromatol Grp, Orense 32004, Spain

年份：2022

卷号：5

起止页码：1176

外文期刊名：CURRENT RESEARCH IN FOOD SCIENCE

收录：SCI-EXPANDED(收录号：WOS:000874870700002)、、Scopus(收录号：2-s2.0-85135388697)、WOS

基金：This research was supported by the National Natural Science Foundation of China (32072212) ) , China, the Research Fund of the University of Macau (SRG2019-00154-ICMS, MYRG2018-00169-ICMS) ) , Macau SAR, China, the Macao Science and Technology Devel-opment Fund, Macau SAR (0098/2020/A, 0024/2020/A1) ) , Macau SAR, China, the 2020 Guangdong Provincial Science and Technology Innovation Strategy Special Fund (Guangdong-Hong Kong-Macau Joint Lab) (2020B1212030006) , Guangdong, China and the State Key Labo-ratory of Quality Research in Chinese Medicine (UM) Internal Research Grant (IRG) (QRCM-IRG2022-008) , Macau SAR, China.

语种：英文

外文关键词：Fisetin; Quercetin; Myricetin; Anti -inflammation; MAPK; Anti-inflammation; NF-?B

外文摘要：Fisetin (Fis), quercetin (Que), and myricetin (Myr) are flavonols with similar structure but different number of hydroxyl groups. The present research focused on the anti-inflammatory effect of these three flavonols in lipopolysaccharide-stimulated RAW264.7 cells. The number and site of hydroxyl group in flavonols obviously affected their anti-inflammation activity. These flavonols suppressed the overproduction of nitric oxide. Fis showed the best activity with an inhibition rate of 52% at 20 mu M. Moreover, the flavonols reduced the levels of ROS, TNF-alpha, and IL-6. The mechanistic study showed that they inhibited the activation of NF-kappa B and MAPK pathways by suppressing the phosphorylation of I kappa B alpha, p65, JNK, ERK, p38, MEK, and reducing the nuclear translocation of NF-kappa B p65. In addition, the metabolism of the flavonols was examined. The results indicated that Fis was both methylated and glucuronidated. Que and Myr were mainly transformed into methylated products. This study highlights the anti-inflammatory activity of flavonols, particularly Fis, which has the potential for the prevention or treatment of inflammation as an adjuvant medicine or food additive.