文献类型：期刊文献

中文题名：壳聚糖/N-乙酰-D-半乳糖胺修饰水飞蓟宾配体脂质体的抗氧化作用研究

英文题名：Antioxidant Effect of Chitosan/N-acetyl-D-galactosamine Modified Silybin Ligand Liposomes

作者：杜卓轩[1];郑品劲[1];孔松芝[1];马柏昂[1];郑心宜[1];黄润华[1];黎展成[1]

机构：[1]广东海洋大学化学与环境学院,广东湛江524088

年份：2023

卷号：50

期号：8

起止页码：57

中文期刊名：广东化工

外文期刊名：Guangdong Chemical Industry

基金：广东省普通高校青年创新人才类项目(230419089);广东海洋大学科研启动经费资助项目(R20034)。

语种：中文

中文关键词：壳聚糖;乙酰半乳糖胺;水飞蓟宾;配体脂质体;抗氧化

外文关键词：chitosan;N-acetyl-D-galactosamine;silybin;liposomes;antioxidation

中文摘要：水飞蓟宾是一种具有抗氧化、抗脂质过氧化作用的成分。本实验采用薄膜分散法制备不同投药量脂质体,并通过滴加法将壳聚糖修饰到脂质体上和通过水合介质将N-乙酰-D-半乳糖胺修饰到脂质体上,以包封率和载药量为主要指标,采用Fenton法测定脂质体清除羟基自由基的适宜浓度和清除率。清除羟基自由基实验结果比较,当浓度为30μg/mL时,脂质体有最大清除率。清除率最高的是投药量为0.0100 g的N-乙酰-D-半乳糖胺修饰的水飞蓟宾脂质体为94%。投药量为0.0080 g的壳聚糖修饰的水飞蓟宾脂质体的包封率93.76%和载药量2.65%为最佳。除壳聚糖修饰的水飞蓟宾脂质体,其他不同修饰的水飞蓟宾脂质体的羟基自由基清除率都随着包封率和载药量的增大而提高。样品间有显著性差异(P<0.05)。

外文摘要：Silybin is an antioxidant and anti-lipid peroxidation component.In this experiment,liposomes with different dosages were prepared by thin-film dispersion method,and chitosan was modified to liposomes by dropping method and N-acetyl-D-galactosamine was modified to liposomes by hydration medium.With encapsulation efficiency and drug loading as the main indexes,Fenton method was used to determine the appropriate concentration and clearance rate of liposomes on the ability to scavenge hydroxyl radicals.The results of scavenging hydroxyl radicals showed that when the concentration was 30μg/mL,the liposomes had the maximum scavenging rate.The highest scavenging rate was 94%for N-acetyl-D-galactosamine modified silybin liposomes with a dosage of 0.0100 g.The encapsulation efficiency(93.76%)and drug loading(2.65%)of chitosan-modified silybin liposomes were the best while the dosage was 0.0080 g.Except for chitosan modified silybin liposomes,the hydroxyl radical scavenging rate of other modified silybin liposomes also increased with the encapsulation efficiency and drug loading.There were significant differences between the samples(P<0.05).