文献类型：期刊文献

英文题名：Establishment and functional characterization of a Nile Tilapia (Oreochromis niloticus) skin epithelial cell line (TSE-04): Applications in transgenics, viral susceptibility, and ecotoxicological stress responses

作者：Chen, Yanghui[1,2];Luo, Junliang[1,2];Jin, Shaoling[1,2];Li, Yuan[3];Huang, Yu[1,2];Cai, Jia[1,2];Jiang, Dongneng[1,2];Jian, Jichang[1,2];Wang, Bei[1,2]

机构：[1]Guangdong Ocean Univ, Fisheries Coll, Guangdong Prov Key Lab Aquat Anim Dis Control & Hl, Zhanjiang, Peoples R China;[2]Key Lab Control Dis Aquat Anim Guangdong Higher Ed, Zhanjiang, Peoples R China;[3]Shaoguan Univ, Henry Fok Coll Biol & Agr, Shaoguan 512005, Peoples R China

年份：2026

卷号：611

外文期刊名：AQUACULTURE

收录：SCI-EXPANDED(收录号：WOS:001542050300003)、、WOS

基金：This work was funded by the project of Ministry of Science and Technology "Belt and Road" innovation talent exchange foreign experts project (DL202303009) , the National Natural Science Foundation of China (Grant No. 32073006, 32002426) , the Natural Science Foundation of Guangdong Province (No. 2022A1515010553) and National Natural Science Foundation of China (No. 320023) .

语种：英文

外文关键词：Virus challenge; Environmental stress; Heavy metal pollution; Skin epithelial cell line

外文摘要：Nile tilapia (Oreochromis niloticus) has become one of the most important fish species in 21st-century aquaculture because of its significant contribution in meeting the growing demand for animal protein. Fish cell lines serve as ideal tools for advancing research in virology and toxicology. As the primary defensive barrier which interfaces directly with external harmful substances, the skin plays a critical role in investigating the effects of various toxic agents on the organism. However, to date, no continuous skin cell line has been developed from O. niloticus. In this study, a novel skin-derived cell line from O. niloticus, exhibiting a typical epithelioid morphology was successfully established, characterized, and designated as TSE-04 cell line. TSE-04 expresses cytokeratin 8, a marker predominantly expressed in epithelial cells. Furthermore, its potential applications in transgenics, virology, and toxicology were demonstrated through successful transfection with the pGFP-N1 plasmid, sensitivity to Singapore grouper iridovirus, and responsiveness to ammonia-nitrogen and heavy-metal stress, respectively. The study also demonstrated that ammonia-nitrogen stress can induce significant oxidative stress and apoptosis in TSE-04. Additionally, chromium (Cr), copper (Cu), zinc (Zn), and manganese (Mn) resulted in varying degrees of cell damage and inflammatory responses in TSE-04. In conclusion, the TSE-04 cell line serves as a good in vitro model for studies in fish virology and toxicology.