文献类型：期刊文献

英文题名：A Sensitive and Validated Method for Determination of T-2 and HT-2 Toxin Residues in Shrimp Tissues by LC-MS/MS

作者：Lu, Pengli[1];Wu, Chaojin[1];Shi, Qi[2];Wang, Yaling[1];Sun, Lijun[1];Liao, Jianmeng[2];Zhong, Saiyi[1];Xu, Defeng[1];Chen, Jinjun[1];Liu, Ying[2];Li, Jianrong[3];Gooneratne, Ravi[4]

机构：[1]Guangdong Ocean Univ, Coll Food Sci & Technol, Guangdong Prov Key Lab Aquat Prod Proc & Safety, Key Lab Adv Proc Aquat Prod,Guangdong Higher Educ, Zhanjiang 524088, Peoples R China;[2]Natl Marine Prod Qual Supervis & Inspect Ctr, Zhanjiang 524000, Peoples R China;[3]Bohai Univ, Food Safety Key Lab Liaoning Prov, Jinzhou 121013, Peoples R China;[4]Lincoln Univ, Ctr Food Res & Innovat, Dept Wine Food & Mol Biosci, Canterbury 7647, New Zealand

年份：2016

卷号：9

期号：6

起止页码：1580

外文期刊名：FOOD ANALYTICAL METHODS

收录：SCI-EXPANDED(收录号：WOS:000375331900013)、、EI(收录号：20154401451365)、Scopus(收录号：2-s2.0-84944908990)、WOS

基金：This study was financially supported by the National Natural Science Foundation of China (NSFC) (No. 31171634 and No. 31371777 and No. 31371746 and No. 31201309) and higher educational cultivation program for major scientific research projects of Guangdong Ocean University (No. GDOU2013050205).

语种：英文

外文关键词：T-2 toxin; HT-2 toxin; LC-MS/MS; Shrimp; Method validation

外文摘要：Accurate analyses of T-2 and HT-2 toxin in aquatic organisms including shrimp are important as these two toxins are increasingly detected in aquatic cereal-based feed. Therefore, the potential for these toxins to enter the human food chain from contaminated fish and shrimp products is very real. A rapid, sensitive, and validated method for simultaneous determination of T-2 and HT-2 toxins was developed using a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method following extraction of the two toxins from shrimp tissues with ethyl acetate. This method is simple in that additional solid-phase extraction is not required to isolate and purify the toxins. LC was performed on an analytical Hypersil GOLD column. The mobile phase consisted of methanol and 5 mM ammonium acetate containing 0.1 % formic acid. The MS/MS ion transitions for both the T-2 toxin (484.20 -> aEuro parts per thousand 214.87) and HT-2 toxin (442.20 -> aEuro parts per thousand 214.96) were monitored. And the most intense transition ion product (m/z) of T-2 and HT-2 used for quantification on the SRM mode of a mass spectrometer was 304.95 and 262.91, respectively. The results linearly correlated with coefficients > 0.9990. The limits of quantification ranged from 0.02 to 0.51 ng center dot g(-1) and from 0.17 to 4.48 ng center dot g(-1) for T-2 and HT-2, respectively, depending on the shrimp tissue type. The overall extraction recovery for both toxins ranged between 84 and 111 % with RSD values less than 15.0 %, indicative of good replication. Furthermore, the recovery and precision levels were within the predefined limits (a parts per thousand currency sign15 %) at all concentrations. The application of this method to study the accumulation of T-2 toxin in shrimp showed that it can be successfully used to monitor even very low tissue toxin concentrations. Research is in progress to extend this method for the measurement of T-2 and HT-2 in aquatic foods that enter the human food chain.