文献类型：期刊文献

英文题名：Roles of chicken growth hormone receptor antisense transcript in chicken muscle development and myoblast differentiation

作者：Xu, H. D.[1];Li, T.[1];Wang, Z.[1];Adu-Asiamah, P.[1];Leng, Q. Y.[1];Zheng, J. H.[1];Zhao, Z. H.[1];An, L. L.[1];Zhang, X. Q.[2];Zhang, L.[1]

机构：[1]Guangdong Ocean Univ, Agr Coll, Zhanjiang 524088, Peoples R China;[2]South China Agr Univ, Minist Agr, Key Lab Chicken Genet Breeding & Reprod, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Guangzhou 510642, Guangdong, Peoples R China

年份：2019

卷号：98

期号：12

起止页码：6980

外文期刊名：POULTRY SCIENCE

收录：SCI-EXPANDED(收录号：WOS:000504411800085)、、Scopus(收录号：2-s2.0-85076064222)、WOS

基金：This work was supported by the National Natural Science Foundation (31672412), the Natural Science Foundation of Guangdong Provincial (2017A030307002), the Open Fund of Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture (Guangzhou) (201501), and The Key Platform Projects of Innovative Strong School Engineering by Department of Education of Guangdong Province (2018302).

语种：英文

外文关键词：chicken; growth hormone receptor; natural antisense transcript; myoblast; differentiation

外文摘要：Muscle is one of the important economic traits in poultry production, and its production depends on the increased number of muscle fibers during the embryonic stage. Chicken GHR gene can transcribe in double directions, possessing not only GHR-S but also GHR-AS. The 2 kinds of transcripts are partially complementation in sequences and interact with each other. Until now, the roles and mechanisms of GHR-AS in myoblast differentiation was still unknown. In this study, we not only analyzed the GHR-AS expression patterns in myoblast differentiation phase but also clarified that GHR-AS promoted myoblast differentiation via GH-GHR-IGF1 signal pathway. Quantitative PCR analysis indicated that GHR-AS was increased during myoblast differentiation. Sub-cellular localization showed that GHR-AS and GHR-S were expressed at a higher level in the nucleus than that in the cytoplasm. The expression of MyoD and MyHC and the myoblast differentiation significantly increased after GHR-AS overexpression, while the distance between wounds decreased, suggesting that GHR-AS repressed myoblast migration and promoted differentiation. Additionally, the expression of GHR-AS, IGF1 and MyHC increased after GH protein treated, and the myoblast differentiation also increased. In conclusion, GHR-AS promoted myoblast differentiation by enhancing fusion and inhibiting migration possibly via GH-GHR-IGF1 signal pathway.