文献类型：期刊文献

英文题名：Transcriptome-wide regulatory networks underlying hypoxic preconditioning-enhanced hypoxia tolerance in the pearl oyster Pinctada fucata martensii

作者：Huang, Jing[1];He, Yilei[1];Yang, Chuangye[1];Luo, Junpeng[1];Wu, Qian[1];Hao, Ruijuan[2];Liao, Yongshan[3,4,5];Wang, Qingheng[1,3,4,5,6];Deng, Yuewen[1,3,4,5,6]

机构：[1]Guangdong Ocean Univ, Fisheries Coll, Zhanjiang 524088, Peoples R China;[2]Southern Marine Sci & Engn Guangdong Lab Zhanjiang, Dev & Res Ctr Biol Marine Resources, Zhanjiang 524006, Peoples R China;[3]Guangdong Ocean Univ, Pearl Res Inst, Zhanjiang, Peoples R China;[4]Pearl Breeding & Proc Engn Technol Res Ctr Guangdo, Zhanjiang 524088, Peoples R China;[5]Guangdong Prov Key Lab Aquat Anim Dis Control & Hl, Zhanjiang 524088, Peoples R China;[6]Guangdong Sci & Innovat Ctr Pearl Culture, Zhanjiang 524088, Peoples R China

年份：2026

卷号：621

外文期刊名：AQUACULTURE

收录：SCI-EXPANDED(收录号：WOS:001746405200001)、、WOS

基金：This research was funded by National Key Research and Development Program of China (Grant no. 2022YFD2401204) , Youth S &T Talent Support Programme of Guangdong Provincial Association for Science and Technology (Grant no. SKXRC2025389) , Shellfish & Algae Industry Innovation Team of Guangdong Modern Agricultural Technology System (Grant no. 2025CXTD23) , Research on industrial inno-vation technology for Guangdong modern marine ranching (Grant no. 2024-MRI-001-03) , and the program for scientific research start-up funds of Guangdong Ocean University (Grant no. 060302022304) . We thank TopEdit ( www.topeditsci.com ) for linguistic assistance during the preparation of this manuscript.

语种：英文

外文关键词：Pinctada fucata martensii; Hypoxic preconditioning; Hypoxia tolerance; miRNA; ceRNA

外文摘要：Hypoxia is a major constraint in aquaculture, and improving tolerance is a practical priority. HPD has been proposed as a strategy to improve hypoxia tolerance in the pearl oyster Pinctada fucata martensii. In this study, we applied HPD to the pearl oyster P. fucata martensii and profiled the regulatory mechanisms by wholetranscriptome sequencing. Oysters in the EG received HPD consisting of eight repeated cycles of moderate hypoxia (DO, 2.0 mg L- 1) for 24 h followed by 48 h recovery under normoxia, while the CG received no HPD. Both groups were then subjected to a 15-day hypoxic challenge (1.5 mg L- 1). HPD preserved gill architecture, with intact filaments, uniform cells, and maintained lacunae. We identified 1165 differentially expressed mRNAs, 676 lncRNAs, 81 circRNAs, and 40 miRNAs, and constructed a ceRNA network comprising 23 lncRNAs, 5 circRNAs, 6 miRNAs, and 10 mRNAs. The network linked the MSTRG.19199.1/novel-m0158-3p axis and the MSTRG.22411.1/miR-363-y axis to modulation of Cu/Zn-SOD and CuAO. It also connected the novel_circ_002334/ novel-m0103-5p axis and the novel_circ_002335/miR-363-y axis to the repression of MED10, with Wnt-associated anti-apoptotic signaling supported by Bcl-2 and ATF6 dynamics. Concomitant suppression of an E3 ubiquitin ligase suggests a key hub coupling hypoxia sensing to immune reprogramming. Additional signatures suggested immune recalibration, metabolic switching involving FAT4 and PDHB, hypoxia-inducible factor 1 alpha related upregulation of PDK1, and reduced transcriptional load through coordinated suppression of HSP70, PIF1, Tcb2, and Dis3L. Together, HPD promotes a pre-adapted, lower-cost homeostatic state that enhances hypoxia tolerance and provides molecular targets for improving oyster resilience in aquaculture.