文献类型：期刊文献

中文题名：基于Illumina MiSeq高通量测序技术分析硇洲岛海星共附生微生物多样性

英文题名：Analysis of symbiotic microorganism diversity in starfish of Naozhou island based on Illumina MiSeq high-throughput sequencing technology

作者：唐慧芳[1];孙力军[1];刘颖[1];陈全毅[1];房志家[1]

机构：[1]广东海洋大学食品科技学院,广东湛江524088

年份：2020

卷号：47

期号：6

起止页码：1675

中文期刊名：微生物学通报

外文期刊名：Microbiology China

收录：CSTPCD、、北大核心2017、CSCD2019_2020、北大核心、CSCD

基金：广东省科技计划项目(2017A030303079,2016A020222014)。

语种：中文

中文关键词：海星;高通量测序技术;共附生微生物;多样性

外文关键词：Starfish;High-throughput sequencing technology;Symbiotic microorganisms;Diversity

中文摘要：【背景】海星作为海洋生物中的一类比较高级的棘皮类动物,其体内蕴藏着丰富且具有生物活性的共附生微生物资源。【目的】分析湛江硇洲岛海星中共附生微生物的多样性。【方法】采用IlluminaMiSeq高通量测序技术分别对硇洲岛海星进行共附生细菌16SrRNA基因V3-V4区和共附生真菌18S rRNA基因ITS1-ITS2区的测序,并根据测序结果进行OTU聚类分析、α多样性分析及物种分类分析等。【结果】高通量测序获得细菌和真菌Filtered的数目分别为61992和71196个,OTU数目分别为2384和529个。经物种分类分析,共附生细菌主要为变形菌门(Proteobacteria),其平均相对含量高达77.37%;其次是厚壁菌门(Firmicutes)、拟杆菌门(Bacteroidetes)、放线菌门(Actinobacteria)和梭杆菌门(Fusobacteria);其中优势细菌属为嗜冷杆菌属(Psychrobacter)和乳球菌属(Lactococcus)。共附生真菌主要为子囊菌门(Ascomycota),其相对含量高达92.33%;其次是霉菌门(Fungi)、担子菌门(Basidiomycota)、被孢菌门(Mortierellomycota)和罗兹菌门(Rozellomycota);优势真菌属以毕赤酵母属(Pichia)为主,未经分类的(unclassified)假丝酵母属(Candida)次之;【结论】硇洲岛海星体内蕴藏着丰富的共附生微生物资源,该研究为今后从事海星微生物资源持续开发和挖掘生物活性物质的研究提供一定的参考依据。

外文摘要：[Background]Starfish is a kind of advanced echinoderm in Marine life,which contains abundant and bioactive resources of Symbiotic microorganisms.[Objective]The diversity of starfish symbiotic microorganisms in Naozhou island of Zhanjiang was analyzed.[Methods]Illumina MiSeq high-throughput sequencing technology was used to sequenced the 16 S rRNA gene V3-V4 region and 18 S rRNA gene ITS1-ITS2 region of bacteria and fungi respectively,and OTUs clustering analysis,Alpha diversity analysis and species classification analysis were conducted according to the sequencing results.[Results]The number of Filtered bacteria and fungi obtained by high-throughput sequencing is 61992 and 71196,and the number of OTUs is 2384 and 529,respectively.According to the classification of species,the common bacteria are mainly Proteobacteria,whose average relative content is up to 77.37%,followed by Firmicutes,Bacteroidetes,Actinobacteria,Fusobacteria.Among dominant genus are Psychrobacter and Lactococcus;Symbiotic Fungi were mainly Ascomycota,with a relative content of 92.33%,followed by Fungi,Basidiomycota,Mortierellomycota and Rozellomycota.The dominant fungi genus was Pichia,followed by unclassified Candida.[Conclusion]There are abundant resources of symbiotic microorganisms in the starfish of Naozhou island,This study provides some reference for the researchers who are engaged in the sustainable development of starfish microbial resources and the excavation of bioactive substances in the future.