文献类型：期刊文献

英文题名：iTRAQ and PRM-based comparative proteomic profiling in gills of white shrimp Litopenaeus vannamei under copper stress

作者：Guo, Hui[1,2,3];Chen, Tianci[1,2,3];Liang, Zhi[1,2,3];Fan, Lanfen[4];Shen, Yuchun[1,2,3];Zhou, Dayan[5]

机构：[1]Guangdong Ocean Univ, Coll Fisheries, Haida Rd 1, Zhanjiang 524025, Peoples R China;[2]Guangdong Higher Educ Inst, Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang, Peoples R China;[3]Guangdong Higher Educ Inst, Key Lab Control Dis Aquat Econ Anim, Zhanjiang, Peoples R China;[4]South China Agr Univ, Coll Marine Sci, Guangzhou 510642, Peoples R China;[5]Aquat Species Intro & Breeding Ctr Guangxi Zhuang, Nanning 530031, Peoples R China

年份：2021

卷号：263

外文期刊名：CHEMOSPHERE

收录：SCI-EXPANDED(收录号：WOS:000595802200320)、、EI(收录号：20203809201491)、Scopus(收录号：2-s2.0-85090992522)、WOS

基金：This research was supported by the National Natural Science Foundation of China (31600321). We are especially grateful for the critical comments from the anonymous reviewers.

语种：英文

外文关键词：Litopenaeus vannamei; iTRAQ; Gill; Cu stress

外文摘要：Crustaceans are particularly sensitive to heavy metal pollution. Copper (Cu) is one of typical heavy metal pollutants in aquatic ecosystems. However, limited attention has been paid on the proteomic responses of shrimp under Cu stress. White shrimp Litopenaeus vannamei held in 5 parts per thousand seawater were exposed to 5 mg L-1 Cu for 3 h, and the regulatory mechanism in the gills was elucidated using iTRAQ-based quantitative proteomics. The results showed that a total of 5034 proteins were identified, 385 differentially expressed proteins (DEPs), including 147 differentially up-regulated proteins (DUPs) and 238 differentially down-regulated proteins (DDPs) were found. Bioinformatics analysis indicated the DEPs responding to Cu stress mainly involved in cytoskeleton, immune response, stress response, protein synthesis, detoxification, ion homeostasis and apoptosis. Furthermore, we still performed PRM analysis on sarcoplasmic calcium binding protein (SCP), serine proteinase inhibitor B3 (SPIB3), C-type lectin 4 (CTL4), cathepsin L (CATHL), JHE-like carboxylesterase 1 (CXE1) and paramyosin (PMY), and biochemical analysis on Cu/Zn-superoxide dismutase (Cu/Zn-SOD) to validate the iTRAQ results, respectively. The present proteome analysis revealed that Cu stress disrupted the ion homeostasis and protein synthesis, and L.vannamei mainly regulates a series of molecular pathways which contained many key proteins involved in the immune process to protect the organism from Cu stress. Our data provides more insight about the underlying mechanisms that related to the stress response of Cu exposure in crustacean. (C) 2020 Elsevier Ltd. All rights reserved.