文献类型：期刊文献

中文题名：矮小体型芦花鸡GHR基因突变类型分析及其与正常体型芦花鸡发育差异研究

英文题名：Analysis of GHR Mutation Types in Dwarf Size Plymouth Rock Chicken(Gallus domesticus) and Study on Its Developmental Difference with the Normal Size One

作者：郑嘉辉[1];冷奇颖[1];张为露[1];Ali Hassan NAWAZ[1];杜炳旺[1];张丽[1]

机构：[1]广东海洋大学滨海农业学院,湛江524088

年份：2021

卷号：29

期号：10

起止页码：1978

中文期刊名：农业生物技术学报

外文期刊名：Journal of Agricultural Biotechnology

收录：CSTPCD、、CSCD2021_2022、北大核心、CSCD、北大核心2020

基金：国家自然科学基金(31672412,31972550);广东省自然科学基金(2020A1515011576)。

语种：中文

中文关键词：矮小体型芦花鸡;正常体型芦花鸡;生长激素受体(GHR)基因;突变类型;品系差异

外文关键词：Dwarf size Plymouth Rock chicken;Normal size Plymouth Rock chicken;Growth hormone receptor(GHR)gene;Mutation type;Strain difference

中文摘要：矮小鸡(Gallus domesticus)由于其体型小,节约养殖空间等优点常被用于鸡配套系生产。本研究为明确矮小体型芦花鸡的生长激素受体(growth hormone receptor, GHR)基因突变类型,探究正常体型芦花鸡和矮小体型芦花鸡间的生长发育差异,针对矮小体型芦花鸡GHR基因常见的4种突变位点设计引物,以矮小体型芦花鸡的基因组DNA为模板进行PCR扩增,分析其遗传变异类型。同时比较分析了正常体型芦花鸡与矮小体型芦花鸡体重和胫长的发育特点,使用Logistic、Gompertz和Von Bertalanffy 3种数学模型拟合分析了其最适模型和拟合参数。结果显示,与正常体型芦花鸡相比,矮小体型芦花鸡GHR基因缺失了1 781 bp的碱基序列。正常体型芦花鸡与矮小体型芦花鸡在1~13周龄期间发育差异明显,尤其在体重和胫长2个性状对比明显。Logistic是正常体型芦花鸡与矮小体型芦花鸡体重的最优拟合模型(R^(2)>0.990)。对于胫长而言,在雄性芦花鸡中,正常体型芦花鸡的最佳拟合模型为Logistic,矮小体型芦花鸡的最适拟合模型为Von Bertalanffy。在雌性芦花鸡中,正常体型芦花鸡和矮小体型芦花鸡的最佳拟合模型均为Von Bertalanffy。本研究为芦花鸡精细饲养管理和矮小芦花鸡的分子标记辅助育种提供了参考依据。

外文摘要：Due to its small size and saving breeding space, dwarf chickens(Gallus domesticus) are often used in the production of chicken supporting lines. The purpose of this study was to clarify the type of growth hormone receptor(GHR) gene mutations in dwarf size Plymouth Rock chicken and to explore the differences in growth and development between the normal size Plymouth Rock chicken and the dwarf size Plymouth Rock chicken. Primers were designed for the four common mutation sites of GHR gene in dwarf size Plymouth Rock chickens. PCR amplification was performed using gDNA of dwarf size Plymouth Rock chickens as templates to analyze the genetic variation types. At the same time, the developmental characteristics of body weight and tibia length of normal and short size Plymouth Rock chickens were compared and analyzed. Three mathematical models of Logistic, Gompertz and Von Bertalanffy were used to analyze the optimal model and fitting parameters. The results showed that compared with the normal size Plymouth Rock chicken, the 1 781 bp deletion of the GHR gene in the dwarf sized Plymouth Rock chicken was the reason for its dwarfing.Normal and dwarf size Plymouth Rock chickens have obvious differences in development during 1 to 13 weeks of age, especially in body weight and tibia length. Logistic was the best fit model for body weight of normal and dwarf size Plymouth Rock chickens(R^(2)>0.990). In terms of tibia length, among male Plymouth Rock chickens, the best fit model for normal sized Plymouth Rock chickens was Logistic, and the best fit model for dwarf sized Plymouth Rock chicken was Von Bertalanffy. Among female Plymouth Rock chickens,the best fit model for normal and dwarf sized Plymouth Rock chickens was Von Bertalanffy. This study provides a reference basis for fine breeding management of Plymouth Rock chickens and molecular markerassisted breeding of the dwarf size Plymouth Rock chickens.