文献类型：期刊文献

英文题名：Cadmium-chelating ability of the siderophore DHBS secreted by Leclercia adecarboxylata FCH-CR2 and its action mechanism

作者：Guan, Wenhao[1];Fang, Zhijia[1];Chen, Yinyan[1];Li, Yongbin[1];Peng, Zhilan[2];Sun, Lijun[1];Deng, Qi[1];Gooneratne, Ravi[3]

机构：[1]Guangdong Ocean Univ, Coll Food Sci & Technol, Guangdong Prov Key Lab Aquat Prod Proc & Safety, Guangdong Prov Engn Technol Res Ctr Marine Food,Ke, Zhanjiang 524088, Peoples R China;[2]Guangdong Med Univ, Marine Biomed Res Inst, Zhanjiang 524023, Peoples R China;[3]Lincoln Univ, Dept Wine Food & Mol Biosci, Lincoln 7647, Canterbury, New Zealand

年份：2023

卷号：900

外文期刊名：SCIENCE OF THE TOTAL ENVIRONMENT

收录：SCI-EXPANDED(收录号：WOS:001054052700001)、、EI(收录号：20233114484437)、Scopus(收录号：2-s2.0-85166290129)、WOS

基金：Acknowledgements This work was supported by the National Natural Science Foundation of China (No. 32172215) , the GuangDong Basic and Applied Basic Research Foundation (No. 2021A1515012443) ,

语种：英文

外文关键词：Cadmium reduction; Leclercia adecarboxylata; Siderophore; 3-dihydroxy-N-benzoyl; L -serine; Cadmium chelating

外文摘要：As one of the most accumulative toxic heavy metals, cadmium (Cd) poses a major threat to human health. Bacterial siderophores, as small molecules with metal-absorbing ability, have great potential activity for Cd-reduction. In this study, the siderophore-producing bacterialstrain FCH-CR2 was isolated from a high-Cd contaminated soil using the CAS method. Leclercia adecarboxylata was identified through 16S rRNA sequence, homology analysis, colony morphology, physiological and biochemical tests. A siderophore, catechol type 2,3-dihydroxy-N-benzoyl-L-serine (DHBS) secreted by FCH-CR2, was purified using RP-HPLC and identified by LC-MS/MS. Intraperitoneal injection of DHBS significantly increased fecal Cd levels, and reduced Cd accumula-tion in organs. In density flooding theory (DFT) analysis, DHBS may bind to Cd via the hydroxyl site on the benzene ring. Besides, the isothermal titration calorimetry (ITC) assay revealed that the formation of Cd-DHBS is a spontaneous and endothermic reaction with & UDelta;G =-21.4 kJ/mol and & UDelta;H = 1.51 & PLUSMN; 0.142 kJ/mol.