文献类型：期刊文献

英文题名：Effects of bisphenol AF on growth, behavior, histology and gene expression in marine medaka (Oryzias melastigma)

作者：Chen, Yuebi[1,2];Chen, Xiaotian[3];Li, Xueyou[1];Liu, Yue[1];Guo, Yusong[1];Wang, Zhongduo[1,4];Dong, Zhongdian[1,2]

机构：[1]Guangdong Ocean Univ, Key Lab Aquaculture South China Sea Aquat Econ Ani, Coll Fishery, Guangdong Higher Educ Inst, Zhanjiang, Peoples R China;[2]Guangdong Ocean Univ, Coll Fishery, Guangdong Prov Key Lab Aquat Anim Dis Control & Hl, Zhanjiang, Peoples R China;[3]Guangdong Acad Sci, Ctr Ind Anal & Testing, Guangzhou 510650, Peoples R China;[4]Hunan Normal Univ Sch, Coll Life Sci, State Lab Dev Biol Freshwater Fish, Changsha, Peoples R China

年份：2022

卷号：308

外文期刊名：CHEMOSPHERE

收录：SCI-EXPANDED(收录号：WOS:000884398300003)、、EI(收录号：20223912812325)、Scopus(收录号：2-s2.0-85138775322)、WOS

基金：This research was funded by the National Natural Science Foundation of China (41806195, 41907346, 31972794), Special Innovation Projects of Colleges and Universities in Guangdong province (2018KTSCX087, 2018KQNCX110), Nanhai Scholar Project of GDOU (QNXZ201807, 201903), and State Laboratory of Developmental Biology of Freshwater Fish (2020KF004).

语种：英文

外文关键词：Bisphenol AF; Oryzias melastigma; Liver toxicity; Lipid metabolism; Antioxidant and inflammatory response; Hypothalamic -pituitary -gonadal axis

外文摘要：Bisphenol AF (BPAF) is one of the substitutes for bisphenol A (BPA), which has endocrine-disrupting, repro-ductive and neurological toxicity. BPAF has frequently been detected in the aquatic environment, which has been a long-term threat to the health of aquatic organisms. In this study, female marine medaka (Oryzias melastigma) were exposed to 6.7 mu g/L, 73.4 mu g/L, and 367.0 mu g/L BPAF for 120 d. The effects of BPAF on behavior, growth, liver and ovarian histology, gene transcriptional profiles, and reproduction of marine medaka were determined. The results showed that with the increase of BPAF concentration, the swimming speed of female marine medaka showed an increasing trend and then decreasing trend. BPAF (367.0 mu g/L) significantly increased body weight and condition factors in females. BPAF (73.4 mu g/L and 367.0 mu g/L) significantly delayed oocyte maturation. Exposure to 367.0 mu g/L BPAF showed an increasing trend in the transcript levels of lipid synthesis and transport -related genes such as fatty acid synthase (fasn), sterol regulatory element binding protein (srebf), diacylglycerol acyltransferase (dgat), solute carrier family 27 member 4 (slc27a4), fatty acid-binding protein (fabp), and peroxisome proliferator-activated receptor gamma (ppar gamma) in the liver. In addition, 6.7 mu g/L BPAF significantly down-regulated the expression levels of antioxidant-related genes [superoxide dismutase (sod), glutathione peroxidase (gpx), and catalase (cat)], and complement system-related genes [complement component 5 (c5), complement component 7a (c7a), mannan-binding lectin serine peptidase 1 (masp1), and tumor necrosis factor (tnf)] were significantly up-regulated in the 73.4 and 367.0 mu g/L groups, which implies the effect of BPAF on the immune system in the liver. In the hypothalamic-pituitary-ovarian axis (HPG) results, the transcription levels of estrogen receptor a (er alpha), estrogen receptor ll (er beta), androgen receptor (ar alpha), gonadotropin-releasing hormone 2 (gnrh2), cytochrome P450 19b (cyp19b), aromatase (cyp19a), and luteinizing hormone receptor (lhr) in the brain and ovary, and vitellogenin (vtg) and choriogenin (chg) in the liver of 367.0 mu g/L BPAF group showed a downward trend. In addition, exposure to 367.0 mu g/L BPAF for 120 d inhibited the spawning behavior of marine medaka. Our results showed that long-term BPAF treatment influenced growth (body weight and condition factors), lipid metabolism, and ovarian maturation, and significantly altered the immune response and the transcriptional expression levels of HPG axis-related genes.