文献类型：期刊文献

中文题名：Isolation and characterization of bioactive fungi from shark Carcharodon carcharias' gill with biopharmaceutical prospects

英文题名：Isolation and characterization of bioactive fungi from shark Carcharodon carcharias' gill with biopharmaceutical prospects

作者：张翼[1,2,3,4];韩金媛[2];冯妍[1];穆军[5];鲍海燕[2];Andreas KULIK[6];Stephanie GROND[4]

机构：[1]College of Food Science and Technology,Guangdong Ocean University,Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety,Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution;[2]School of Environmental and Chemical Engineering,Dalian Jiaotong University;[3]School of Life Science and Biotechnology,Dalian University of Technology;[4]Institute of Organic Chemistry,University of Tübingen;[5]Marine Science and Technology College,Zhejiang Ocean University;[6]Inter-Faculty Institute for Microbiology and Infectious Medicine,University of Tübingen

年份：2016

卷号：34

期号：1

起止页码：186

中文期刊名：Chinese Journal of Oceanology and Limnology

外文期刊名：中国海洋湖沼学报（英文版）

收录：CSTPCD、、CSCD2015_2016、Scopus、CSCD

基金：Supported by the National Natural Science Foundation of China (No. 20902009), the National Science Foundation for Post-Doctoral Scientists of China (Nos. 2011M500051, 2012T50258), the YangFan Scarce Top Talent Project of Guangdong Province (to ZHANG Yi), the Program for Scientific Research Start-up Funds of Guangdong Ocean University (GDOU) (to ZHANG Yi), and the Natural Science Research Project of GDOU (No. C14519)

语种：中文

中文关键词：bioactive fungi; shark gill; isolation; bioassay; taxonomy; bioactive metabolites

外文关键词：生物活性菌;分离鉴定;生物制药;鱼鳃;鲨鱼;DNA序列比对;生物活性化合物;遗传多样性

中文摘要：Until recently, little was known about the fungi found in shark gills and their biomedicinal potential. In this article, we described the isolation, bioactivity, diversity, and secondary metabolites of bioactive fungi from the gill of a shark （Carcharodon carcharias）. A total of 115 isolates were obtained and grown in 12 culture media. Fifty-eight of these isolates demonstrated significant activity in four antimicrobial, pesticidal, and cytotoxic bioassay models. Four randomly selected bioactive isolates inhibited human cancer cell proliferation during re-screening. These active isolates were segregated into 6 genera using the internal transcribed spacer-large subunit （ITS-LSU） rDNA-sequence BLAST comparison. Four genera, Penicillium, Aspergillus, Mucor, and Chaetomium were the dominant taxa. A phylogenic tree illustrated their intergenera and intragenera genetic diversity. HPLC-DAD-HRMS analysis and subsequent database searching revealed that nine representative strains produced diverse bioactive compound profiles. These results detail the broad range of bioactive fimgi found in a shark＇s gills, revealing their biopharmaceutical potential. To the best of our knowledge, this is the first study characterizing shark gill fungi and their bioactivity.

外文摘要：Until recently,little was known about the fungi found in shark gills and their biomedicinal potential. In this article,we described the isolation,bioactivity,diversity,and secondary metabolites of bioactive fungi from the gill of a shark(Carcharodon carcharias). A total of 115 isolates were obtained and grown in 12 culture media. Fifty-eight of these isolates demonstrated significant activity in four antimicrobial,pesticidal,and cytotoxic bioassay models. Four randomly selected bioactive isolates inhibited human cancer cell proliferation during re-screening. These active isolates were segregated into 6 genera using the internal transcribed spacer-large subunit(ITS-LSU) rDNA-sequence BLAST comparison. Four genera,Penicillium,Aspergillus,Mucor,and Chaetomium were the dominant taxa. A phylogenic tree illustrated their intergenera and intragenera genetic diversity. HPLC-DAD-HRMS analysis and subsequent database searching revealed that nine representative strains produced diverse bioactive compound profiles. These results detail the broad range of bioactive fungi found in a shark's gills,revealing their biopharmaceutical potential. To the best of our knowledge,this is the first study characterizing shark gill fungi and their bioactivity.