文献类型：期刊文献

英文题名：miR-10a-3p Participates in Nacre Formation in the Pearl Oyster Pinctada fucata martensii by Targeting NPY

作者：Yang, Min[1];Li, Xin lei[1];Zhang, Yu ting[1];Deng, Yue wen[1,2,3,4];Jiao, Yu[1,2,3]

机构：[1]Guangdong Ocean Univ, Fishery Coll, Zhanjiang 524025, Peoples R China;[2]Pearl Breeding & Proc Engn Technol Res Ctr Guangdo, Zhanjiang 524088, Peoples R China;[3]Guangdong Sci & Innovat Ctr Pearl Culture, Zhanjiang 524088, Peoples R China;[4]Guangdong Prov Key Lab Aquat Anim Dis Control & Hl, Zhanjiang 524088, Peoples R China

年份：2023

卷号：25

期号：3

起止页码：428

外文期刊名：MARINE BIOTECHNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000994890200001)、、EI(收录号：20232214168251)、Scopus(收录号：2-s2.0-85160452344)、WOS

语种：英文

外文关键词：miR-10a-3p; Neuropeptide Y; Pinctada fucata martensii; Nacre; Biomineralization

外文摘要：MicroRNAs (miRNAs) are small noncoding RNAs that regulate gene expression via the recognition of their target messenger RNAs. MiR-10a-3p plays an important role in the process of ossification. In this study, we obtained the precursor sequence of miR-10a-3p in the pearl oyster Pinctada fucata martensii (Pm-miR-10a-3p) and verified its sequence by miR-RACE technology, and detected its expression level in the mantle tissues of the pearl oyster P. f. martensii. Pm-nAChRs alpha and Pm-NPY were identified as the potential target genes of Pm-miR-10a-3p. After the over-expression of Pm-miR-10a-3p, the target genes Pm-nAChRs alpha and Pm-NPY were downregulated, and the nacre microstructure became disordered. The Pm-miR-10a-3p mimic obviously inhibited the luciferase activity of the 3 ' untranslated region of the Pm-NPY gene. When the interaction site was mutated, the inhibitory effect disappeared. Our results suggested that Pm-miR-10a-3p participates in nacre formation in P. f. martensii by targeting Pm-NPY. This study can expand our understanding of the mechanism of biomineralization in pearl oysters.