详细信息
文献类型:期刊文献
中文题名:DNA技术在海洋食品物种鉴定中的应用
英文题名:DNA-based methods for identification of seafood species
作者:张丽[1];张良[2];刘书成[2];张义军[3];韩艺[4]
机构:[1]广东海洋大学农学院,湛江524025;[2]广东海洋大学食品科技学院,湛江524025;[3]河南省内黄县安乡农业服务中心,安阳456300;[4]河南省鹿邑县畜牧局,周口477200
年份:2010
卷号:32
期号:6
起止页码:555
中文期刊名:遗传
外文期刊名:Hereditas(Beijing)
收录:CSTPCD、、Scopus、CSCD2011_2012、北大核心2008、北大核心、CSCD、PubMed
基金:广东海洋大学博士启动基金项目(编号:0712130)资助
语种:中文
中文关键词:DNA技术;海洋食品;物种鉴定
外文关键词:DNA biotechnology; seafood; species identification
中文摘要:随着分子生物学技术的发展,海洋食品物种鉴定方法由原来的蛋白质水平深入到了DNA水平。目前应用于海洋食品物种鉴定的DNA技术主要是FINS(Forensically informative nucleotide sequencing)、PCR-RFLP和物种特异性PCR标记技术等,能够实现对新鲜、冰冻、腌制或灌装食品物种进行鉴定,而对混合样本的鉴定及量化分析是尚待解决的一个问题。基因数据库对物种鉴定的影响也越来越大,是海洋加工食品物种鉴定可利用的另一种重要信息资源。文章综述了DNA技术在海洋食品物种鉴定中的应用研究进展,并展望DNA技术在海洋食品检测中的发展趋势。
外文摘要:With the development of molecular biotechnology, methods for identification of seafood species are developed from protein to DNA. At present, the main DNA-based methods for species identification are FINS, PCR-RFLP, and specific-PCR, which have been used to identify the species of fresh, frozen, and pickled or canned seafood. However, qualitative and quantitative methods for identification of the mixed seafood species remain to be resolved. The gene databases play an important role in identifying species and are valuable information resources for identification of seafood species. In this paper, recent progresses of major DNA-based methods for identification of seafood species are reviewed and the perspectives of this field are discussed.
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