文献类型：期刊文献

英文题名：Extraction, purification, and structure characterization of polysaccharides from Crassostrea rivularis

作者：Qin, Xiaoming[1,2,3,4];Fan, Xiuping[2,4];Zhang, Lianyi[4];Zheng, Huina[1,2,3,4];Zhang, Chaohua[1,2,3,4];Yuan, Jianjun[5]

机构：[1]South China Sea Bioresource Exploitat & Utilizat, Zhanjiang 524088, Peoples R China;[2]Guangdong Prov Key Lab Aquat Prod Proc & Safety, Zhanjiang, Peoples R China;[3]Natl Res & Dev Branch Ctr Shellfish Proc, Zhanjiang, Peoples R China;[4]Guangdong Ocean Univ, Coll Food Sci & Technol, Zhanjiang, Peoples R China;[5]Fujian Prov Key Lab Dev Bioact Mat Marine Algae, Quanzhou 362000, Peoples R China

年份：2018

卷号：6

期号：6

起止页码：1621

外文期刊名：FOOD SCIENCE & NUTRITION

收录：SCI-EXPANDED(收录号：WOS:000446166200032)、、WOS

基金：special funds for the Construction of Modern Agricultural Industrial Technology System, Grant/Award Number: CRS-49

语种：英文

外文关键词：chemical characterization; Crassostrea rivularis; glycogen; polysaccharide

外文摘要：Crude polysaccharide was prepared from Crassostrea rivularis by 30% (w/v) potassium hydroxide solution at 90 degrees C for 120min. Three fractions (OG1, OG2, and OG3) were purified by DEAE-52 cellulose and Sepharose 2B gel column chromatography. The chemical structures were determined using gas chromatography (GC), high-performance gel permeation chromatography (HPGPC), Fourier-transform infrared (FT-IR) spectroscopy, and H-1 and C-13 nuclear magnetic resonance (NMR) spectroscopy. The results indicated that OG1 was composed of rhamnose and little mannose (8.71%), the ratio of Rha: Gal: Xyl: Fuc in OG3 were 14:5.5:3:1. And their average molecular weights (Mw) were about 1.66x10(6) and 2.33x10(6)Da, respectively. OG2 was composed only of glucose (98.23%), which means it was glycogen. OG2 was consisted mainly of 4)- -D-Glc-(1, with the branch chain every 6.5 glucose residues on average, which is 4,6)--D-Glc-(1 and trace amount of -D-Glc-(1 branched units. The Mw was 2.27x10(6)Da. It provides the bases for the bioactivity research.