文献类型：期刊文献

中文题名：对虾中隐蔽态T-2毒素残留对小鼠血常规和血清生化指标的影响

英文题名：Effect of masked T-2 toxin residues in Litopenaeus vannamei on routine blood and serum biochemical indexes of mice

作者：黄展锐[1];王雅玲[1];王小博[1];莫冰[2];邱妹[1];孙力军[1];陈康健[3];刘健[3]

机构：[1]广东海洋大学食品科技学院广东省水产品加工与安全重点实验室广东普通高等学校水产品深加工重点实验室;[2]广西壮族自治区产品质量监督检验研究院;[3]湛江恒兴水产科技有限公司

年份：2017

卷号：29

期号：7

起止页码：1110

中文期刊名：浙江农业学报

外文期刊名：Acta Agriculturae Zhejiangensis

收录：CSTPCD、、北大核心2014、CSCD2017_2018、北大核心、CSCD

基金：国家自然科学基金面上项目(31171634;31371777);广东省科技计划项目(2014B020205006)

语种：中文

中文关键词：对虾;隐蔽态T-2毒素;血常规;血清

外文关键词：shrimp; masked T-2 toxin; routine blood; serum

中文摘要：T-2毒素经20 d蓄积毒性试验法染毒对虾,采用LC-MS/MS检测对虾组织中游离态T-2毒素,并将染毒的虾头和肌肉匀浆液连续灌胃小鼠7 d,检测小鼠血常规和血清指标,分析对虾虾头和肌肉中隐蔽态T-2毒素残留对小鼠的毒性效应。结果显示,经20 d蓄积毒性试验,对虾体内没有游离态T-2存在,而且低剂量染毒对虾对小鼠没有影响,但高剂量组(12.2 mg·kg^(-1))可引起小鼠中间粒细胞增加,红细胞系统和血小板各项指标均显著下降(P<0.05),血清生化指标差异显著(P<0.05),且虾头对小鼠的损伤作用高于肌肉。研究表明,蓄积染毒对虾体内存在隐蔽态T-2毒素,对小鼠具有一定的免疫毒性和血液毒性,导致炎症发生、贫血以及骨髓造血功能下降,对肝功能有损伤作用,且虾头中残留的隐蔽态T-2毒素对小鼠的损伤作用高于肌肉。

外文摘要：After 20 d accumulation toxicity test of T-2 toxin, the free T-2 toxin in shrimp was quantitatively detected using LC-MS/MS. The shrimp head and muscle homogenate were administered to mice for 7 days, the blood routine and serum indexes in mice were detected to study the toxicity effect of residual masked T-2 toxin in Litopenaeus van-namei head and muscle on routine blood and serology of mice. The results showed that there was no free T-2 toxin in shrimp during 20 d accumulation toxicity test of T-2 toxin. And low-dose shrimp had no effect on mice. However, the high dose group shrimp caused an increase in neutrophils in mice, and erythrocyte system and platelet were significantly decreased （ P 〈 0. 05 ） . Moreover, serum biochemical indicators were significantly different （ P 〈 0. 05 ） . The damage of the head to the mice was higher than that of the muscle. Research showed that there was masked T-2 toxin in the infected shrimp, which had immune toxicity and blood toxicity to mice and led to the occurrence of inflamma-tion, symptoms of anaemia, reduced the bone marrow hematopoietic function and damaged liver function. The dam-age of residual masked T-2 toxin in the head to the mice was higher than that of the muscle.