文献类型：期刊文献

英文题名：Effect of Oral Administration of Active Peptides of Pinctada Martensii on the Repair of Skin Wounds

作者：Yang, Faming[1];Qin, Xiaoming[1,2,3,4,5,6];Zhang, Ting[1];Zhang, Chaohua[1,2,3,4,5,6];Lin, Haisheng[1,2,3,4,5,6]

机构：[1]Guangdong Ocean Univ, Coll Food Sci & Technol, Zhanjiang 524088, Peoples R China;[2]Natl Res & Dev Branch Ctr Shellfish Proc Zhanjian, Zhanjiang 524088, Peoples R China;[3]Guangdong Prov Key Lab Aquat Prod Proc & Safety, Zhanjiang 524088, Peoples R China;[4]Guangdong Prov Engn Lab Marine Biol Prod, Zhanjiang 524088, Peoples R China;[5]Key Lab Adv Proc Aquat Prod Guangdong Higher Educ, Zhanjiang 524088, Peoples R China;[6]South China Sea Bioesource Exploitat & Utilizat C, Zhanjiang 524088, Peoples R China

年份：2019

卷号：17

期号：12

外文期刊名：MARINE DRUGS

收录：SCI-EXPANDED(收录号：WOS:000507340000049)、、WOS

基金：This research was funded by the National Modern Agricultural Industry Technology System (CARS-49), Guangdong Province Applied Science and Technology R&D Special Fund Project (2016B020235002), Guangdong Ocean University Graduate Education Innovation Program Excellent Dissertation Cultivation Project (521005036), High Value of Aquatic Products in Guangdong's Ordinary Higher Education Institutions Processing and Utilization Innovation Team Project (GDOU2016030503).

语种：英文

外文关键词：Pinctada martensii; mantle; active peptides; skin; wound healing

外文摘要：Skin wound healing, especially chronic wound healing, is a common challenging clinical problem. It is urgent to broaden the sources of bioactive substances that can safely and efficiently promote skin wound healing. This study aimed to observe the effects of active peptides (APs) of the mantle of Pinctada martensii on wound healing. After physicochemical analysis of amino acids and mass spectrometry of APs, the effect of APs on promoting healing was studied through a whole cortex wound model on the back of mice for 18 consecutive days. The results showed that APs consisted of polypeptides with molecular weights in the range 302.17-2936.43 Da. The content of polypeptides containing 2-15 amino acids accounted for 73.87%, and the hydrophobic amino acids accounted for 56.51%. Results of in vitro experimentation showed that mice in APs-L group which were fed a low dose of APs (0.5 g/kg bw) had a shortened epithelialization time due to a shortening inflammatory period (p < 0.05). Mechanistically, this relied on its specific ability to promote the proliferation of CD31, FGF and EGF which accelerated the percentage of wound closure. Moreover, the APs-L group mice had enhanced collagen synthesis and increased type III collagen content in their wounds through a TGF-beta/Smad signaling pathway (p > 0.05). Consequently, scar formation was inhibited and wound healing efficiency was significantly improved. These results show that the APs of Pinctada martensii promote dermal wound healing in mice and have tremendous potential for development and utilization in skin wound healing.