文献类型：期刊文献

中文题名：火龙果溃疡病菌拮抗细菌的鉴定和发酵培养基优化

英文题名：Identification of Antagonistic Bacterium against Neoscytalidium dimidiatum Causing Pitaya Canker and Fermentation Medium Optimization

作者：陈静[1];李少梅[1];许小玲[1];苏桐洁[1];许耀杰[1];邢益原[2];易润华[1]

机构：[1]广东海洋大学农学院生物技术系,广东湛江524088;[2]徐闻绿源果菜发展有限公司,广东徐闻524100

年份：2015

卷号：35

期号：10

起止页码：64

中文期刊名：热带农业科学

外文期刊名：Chinese Journal of Tropical Agriculture

收录：CSTPCD

基金：广东海洋大学2014年大学生科技创新培育资助(No.校团委[2014]9号D62)

语种：中文

中文关键词：火龙果溃疡病;皮氏类芽孢杆菌;拮抗菌;培养基优化

外文关键词：pitaya canker;Paenibacillus peoriae;antagonistic bacteria;medium optimization

中文摘要：采用对峙培养法从来自土壤的136株细菌中筛选得到对火龙果溃疡病菌有拮抗作用的菌株HTN-5,抑菌带宽9.0 mm。通过形态学、生理生化特性和16s r DNA序列分析,将拮抗菌HTN-5初步鉴定为皮氏类芽孢杆菌Paenibacillus peoriae。从8种培养基中筛选出IFFI培养基最有利于菌株HTN-5形成抑菌活性物质,正交试验优化其成分,得最优配方为:牛肉膏2.50 g/L,蛋白胨10.00 g/L,葡萄糖12.50 g/L,乳糖10.00 g/L,酵母膏2.50 g/L,氯化钠5.00g/L,p H 6.8。

外文摘要：The isolate HTN-5, which antagonized against Neoscytalidium dimidiatum causing pitaya canker, was screened from one hundred and thirty-six bacteria which isolated from the diseased orchard soil. The width of inhibiting zone between HTN-5 and Neoscytalidium dimidiatum reached 9.0 mm. HTN-5 was identified as Paenibacillus peoriae based on morphology, physio-biochemical reactions and 16s rDNA sequences. The IFFI medium was the most suitable medium for isolate HTN-5 to produce antimicrobial active substances, and the optimal medium ingredients, was obtained by the L16 （4^5） Latin orthogonal experiments, which were comprised of beef extract 2.50 g/L, peptone 10.00 g/L glucose 12.50 g/L, lactose 10.00 g/L, yeast extract 2.50 g/L, sodium chloride 5.00 g/L, pH6.8.