文献类型：期刊文献

英文题名：Zein-Paclitaxel Prodrug Nanoparticles for Redox-Triggered Drug Delivery and Enhanced Therapeutic Efficiency

作者：Hou, Heting[1,2];Zhang, Dong[3];Lin, Jiewen[1,2];Zhang, Yingying[4];Li, Chengyong[5];Wang, Zhe[6];Ren, Jiaoyan[7];Yao, Maojin[8];Wong, Ka-hing[3];Wang, Yi[1,2,3]

机构：[1]Shenzhen Res Inst Hong Kong Polytech Univ, State Key Lab Chinese Med & Mol Pharmacol Incubat, Shenzhen 518057, Peoples R China;[2]Shenzhen Res Inst Hong Kong Polytech Univ, Shenzhen Key Lab Food Biol Safety Control, Shenzhen 518057, Peoples R China;[3]Hong Kong Polytech Univ, Dept Appl Biol & Chem Technol, Kowloon, Hong Kong, Peoples R China;[4]Cent S Univ, Dept Radiat Oncol, Xiangya Hosp, Changsha 410008, Hunan, Peoples R China;[5]Guangdong Ocean Univ, Sch Chem & Environm, Zhanjiang 524088, Peoples R China;[6]Chinese Acad Sci, Ctr Biomed Mat & Interfaces, Shenzhen Inst Adv Technol, Shenzhen 518055, Peoples R China;[7]South China Univ Technol, Sch Food Sci & Engn, Wushan Rd, Guangzhou, Guangdong, Peoples R China;[8]Sun Yat Sen Univ, Dept Anesthesiol, Sun Yat Sen Mem Hosp, 107 Yan Jiang Xi Rd, Guangzhou 510120, Guangdong, Peoples R China

年份：2018

卷号：66

期号：44

起止页码：11812

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：SCI-EXPANDED(收录号：WOS:000449888000031)、、EI(收录号：20184606061448)、Scopus(收录号：2-s2.0-85056247654)、WOS

基金：We thank the State Key Laboratory of Chinese Medicine and Molecular Pharmacology (Incubation) and Shenzhen Key Laboratory of Food Biological Safety Control for their support. This work is financially supported by the Shenzhen Basic Research (Layout of Disciplines) Project Fund (JCYJ20170413154810633), the General Research Fund (GRF) of Hong Kong (PolyU 153343/16P), the Health and Medical Research Fund (HMRF) of the Food and Health Bureau of Hong Kong (03144126, 05161016), and Central Research Fund of the Hong Kong Polytechnic University (4-BCA8, G-UA4C, G-YBJ7, and G-YBU1). We appreciate the help from the Material Research Center of the Hong Kong Polytechnic University.

语种：英文

外文关键词：prodrug; self-assembly; redox-responsive; zein; triggered release; paclitaxel

外文摘要：Prodrug, in which the inactive parent drug with good bioavailability is metabolized into an active drug in the body, is one of the main strategies to target the disease site to improve the drug efficiency and reduce the adverse effects of chemotherapy. Because of the good capability of chemical modification, zein, a plant derived protein, and drugs can be conjugated through environmentally sensitive links to form prodrugs capable of triggered drug release. In this study, a novel prodrug was synthesized using paclitaxel (PTX), zein, and a disulfide linker, and nanoparticles were formed by self-assembly of the prodrug. An effective in vitro triggered release, 80-90% in 5 min, of the prodrug based nanoparticles (zein-S-S-PTX_NP) was successfully approached. The cytotoxicity of zein-S-S-PTX_NP as well as the zein encapsulation of PTX (zein_PTX_NP) and pure PTX on HeLa cells and NIH/3T3 fibroblast cells was tested using MTS assay. It showed that, after the treatment of zein-S-S-PTX_NP at the equivalent PTX concentrations of 0.1, 0.5, 1, and 5 mu g/mL, respectively, zein-S-S-PTX_NP had zero damage to normal cells but a similar cytotoxicity to cancer cells as pure PTX. In the animal study, the tumor was 50% of the original size after the treatment of zein-S-S-PTX_NP for 9 days with 3 doses. This study suggested that the novel prodrug based nanoparticle zein-S-S-PTX_NP could be a promising approach in chemotherapy with targeted delivery, improved efficacy, and reduced side effects.