文献类型：期刊文献

英文题名：Earthworm-assisted shift and enrichment of the active di(2-ethylhexyl) phthalate degraders in farmland soils by DNA-stable isotope probing

作者：Wei, Ting[1];Zhen, Zhen[1];Luo, Shuwen[1];Chen, Yijie[1];Li, Jin[2];Liang, Yan-Qiu[2];Song, Mengke[3];Li, Jincheng[4];Lin, Zhong[2,5];Zhang, Dayi[6,7,8]

机构：[1]Guangdong Ocean Univ, Coll Coastal Agr Sci, Zhanjiang 524088, Peoples R China;[2]Guangdong Ocean Univ, Fac Chem & Environm Sci, Zhanjiang 524088, Peoples R China;[3]South China Agr Univ, Coll Nat Resources & Environm, Guangzhou 510642, Peoples R China;[4]Huipeng Agroecol Technol Co Ltd, Zhanjiang 524000, Peoples R China;[5]Guangdong Ocean Univ, Shenzhen Res Inst, Shenzhen 518108, Peoples R China;[6]Minist Educ, Key Lab Groundwater Resources & Environm, Changchun 130021, Peoples R China;[7]Jilin Univ, Coll New Energy & Environm, Changchun 130021, Peoples R China;[8]Shenyang Univ, Minist Educ, Key Lab Reg Environm & Ecorestorat, Shenyang 110044, Peoples R China

年份：2025

卷号：498

外文期刊名：JOURNAL OF HAZARDOUS MATERIALS

收录：SCI-EXPANDED(收录号：WOS:001584411500002)、、EI(收录号：20253919227162)、Scopus(收录号：2-s2.0-105016782331)、WOS

基金：This work was financially supported by National Natural Science Foundation of China (41977125, 41907033) , Natural Science Foundation of Guangdong Province (2024A1515010766, 2023A1515012225) , Shenzhen Fundamental Research Program (JCYJ20220818103609020) , Science and Technology Program of Shenyang (23-407-3-09) and Technology Concept Verification Project at Jilin University (2024GN031) .

语种：英文

外文关键词：DEHP; Earthworm gut; Active degraders; Degradation pathways; DNA-stable isotope probing

外文摘要：Di(2-ethylhexyl) phthalate (DEHP) is a widely used plasticizer that poses significant environmental and health risks due to its accumulation in agricultural soils and endocrine-disrupting effects. This study investigated the roles of earthworm-assisted bioremediation of DEHP by using DNA stable isotope probing (DNA-SIP) and metagenomic sequencing, and identified the shift of the active microbes involved in DEHP degradation. DEHP degradation efficiency increased from 41.72 % in natural soil to 79.90 % in earthworm-amended soil, 47.31 % and 9.48 % of which attributed to gut microbiota and improved soil microenvironment. We identified 15 active DEHP-degrading OTUs, including five newly reported genera (Romboutsia, Deinococcus, Paeniglutamicibacter, Intrasporangium, and Methylotenera). Functional pathway analysis revealed microenvironment-specific degrada-tion routes. The 2,3-epoxy-2,3-dihydrobenzoyl-CoA and 4,5-dihydroxyphthalate pathways were exclusively active in soil, while cis,cis-muconate and 2-oxopent-4-enoate pathways were unique in earthworm gut. Notably, both soil and gut shared 4-hydroxyphthalate and glutaryl-CoA pathways, and the latter one was highly promoted in gut (1.11 %) and strongly associated with key degraders such as Pseudomonas, Ensifer, and Acinetobacter. All gut-associated degradation routes converged into the citrate cycle, indicating enhanced potential for complete mineralization under microaerobic conditions. This work provides valuable strategies for optimizing earthworm-assisted bioremediation to mitigate DEHP pollution in agricultural soils.