文献类型：期刊文献

英文题名：Isolation and characterisation of acid-solubilised collagen from the skin of Nile tilapia (Oreochromis niloticus)

作者：Zeng, Shao-kui[2];Zhang, Chao-hua[2];Lin, Hong[1];Yang, Ping[2];Hong, Peng-zhi[2];Jiang, Zhihong[2]

机构：[1]Ocean Univ China, Fac Food Sci & Technol, Qingdao 266033, Shandong, Peoples R China;[2]GuangDong Ocean Univ, Fac Food Technol, Zhanjiang 524025, Guangdong, Peoples R China

年份：2009

卷号：116

期号：4

起止页码：879

外文期刊名：FOOD CHEMISTRY

收录：SCI-EXPANDED(收录号：WOS:000267522700010)、、Scopus(收录号：2-s2.0-67349229111)、WOS

基金：Financial Support was provided by the Guangdong Science and Technology Programme Fundation (2005A20301001). Authors thank Engineer Zheng Jiagai with China National Analytical Center for amino acid analysis, and Zhanjiang Global Aquatic Product Co., Ltd. for providing fish skin.

语种：英文

外文关键词：Collagen; Oreochromis niloticus; Skin; Acid-solubilised collagen (ASC); Solubility; alpha Chain

外文摘要：Acid-solubilised collagen (ASC) was extracted from the skin of Nile tilapia (Oreochromis niloticus) and characterisation was studied. The results indicated that the yield of ASC was 39.4% on the basis of dry weight. This ASC was rich in glycine (35.6%). The amount of imino acids, proline and hydroxyproline, in ASC was 210 residues per 1000 residues. The ultraviolet (UV) absorption spectrum of ASC showed that the distinct absorption was at 220 run. ASC showed transition curve at maximum temperature of 32.0 degrees C in 0.05 M acetic acid, about 12 degrees C lower than that of calf skin collagen. Maximum solubility (0.75 mg/ml) in 0.5 M acetic acid was observed at pH 3. Solubility reached the minimum at pH 7. A sharp decrease in Solubility was observed in 2% (w/v) NaCl or above. Biochemical Studies indicated that ASC was composed of the alpha 1 alpha 2 alpha 3 heterotrimers. (C) 2009 Elsevier Ltd. All rights reserved.