文献类型：期刊文献

中文题名：以D2EHPA为流动载体的乳状液膜萃取菲牛蛭中的水蛭素实验研究

英文题名：Emulsion liquid membrane extraction with D2EHPA mobile carrier Hirudinaria manillensis hirudin in experimental study

作者：方富永[1];苗艳丽[2];刘惠钦[2];何燕君[2];陈绍红[1];廖艳[1];刘少彬[3];宋文东[2];赵云涛[1]

机构：[1]广东海洋大学现代生物化学中心,广东湛江524088;[2]广东海洋大学理学院,广东湛江524088;[3]广东海洋大学农学院,广东湛江524088

年份：2012

卷号：37

期号：20

起止页码：3056

中文期刊名：中国中药杂志

外文期刊名：China Journal of Chinese Materia Medica

收录：MEDLINE(收录号：23311153)、CSTPCD、、北大核心2011、Scopus(收录号：2-s2.0-84873460308)、CSCD2011_2012、北大核心、CSCD、PubMed

基金：广东省省部产学研结合项目(2011B090400252);国家海洋局海洋公益性行业科研专项经费项目(200905021)

语种：中文

中文关键词：乳状液膜;二-(2-乙基已基)磷酸;水蛭素;萃取;载体

外文关键词：emulsion liquid membrane ; di- （2-ethylhexyl） phosphate ; hirudin ; extraction ; carrier

中文摘要：目的:研究以菲牛蛭为原料,二-(2-乙基已基)磷酸(D2EHPA)作流动载体,脱水山梨醇脂肪酸酯(Span 80)为乳化剂,辛烷与D2EHPA混合构成膜溶液,稀HCl溶液作内水相的水蛭素乳状液膜体系萃取。方法:用正交试验优化膜相、内水相体积比(MIPVR),内、外水相的pH,流动载体浓度等水蛭素对照品萃取条件,然后用水蛭素粗提液进行纯化实验。结果:萃取的最优条件为:MIPVR 10∶3,内水相pH 2.6,外水相pH 3.4,流动载体质量分数2%;当水蛭素初始浓度为1抗凝血酶活力单位(ATU).mL-1时,对照品ATU回收率为83.06%,粗提液达到82.99%,样品比活力为3 289.48 ATU.mg-1,不连续聚丙烯酰胺凝胶电泳及光谱扫描结果表明纯度与对照品相当。结论:乳状液膜体系萃取水蛭素工艺较简单,实验样品纯度及ATU回收率均较高。

外文摘要：Objective： To study the extraction system of hirudin emulsion liquid membrane with the Poecilobdella manillensis as raw material, di-（2-ethylhexyl） phosphate（D2EHPA） as carrier, Span 80 as emulsifier, octane and D2EHPA mixed to constitute membrane solution, diluted HC1 solutions as internal aqueous phase. Method： Using the orthogonal experiment to optimize the extrac- tion conditions of hirudin reference substance such as membrane phase, internal aqueous phase volume ratio （MIPVR） ,external aque- ous phase pH, internal aqueous phase pH, mobile carrier concentration and so on, and then using hirudin crude extracts to do purifying experiment, and gaining experimental samples. Result： The optimal conditions of hirudin extraction were as follows： MIPVR 10： 3, in- ternal aqueous phase pH 2. 6, external aqueous phase pH 3.4, the mass fraction of carrier D2EHPA 2%. In the optimal extraction con- ditions, when the initial concentration of hirudin was one anti-thrombin activity units （ATU） ~ mL- ~, ATU recovery rate of the reference substance was 83.06%. In the purifying experiment of crude extracts, ATU recovery rate was 82. 99% , and the specific activity of sam- ple was 3 289.48 the ATU · mg-1. Discontinuous polyacrylamide gel electrophoresis and spectral scanning,the results showed that the purity and reference substance were considerable. Conclusion： The method of preparation hirudin was relatively simple, the purity of the experimental samples and ATU recovery were both high.