文献类型：期刊文献

英文题名：The isolation, identification, whole-genome sequencing of Clostridium butyricum LV1 and its effects on growth performance, immune response, and disease-resistance of Litopenaeus vannamei

作者：Wang, Qi[1,2,3,5];Li, Weikang[3,4];Liu, Hongyu[1,2,3];Tan, Beiping[1,2,3];Dong, Xiaohui[1,2,3];Chi, Shuyan[1,2,3];Yang, Qihui[1,2,3];Zhang, Shuang[1,2,3];Fan, Depeng[5];Hu, Yadong[5]

机构：[1]Guangdong Ocean Univ, Fisheries Coll, Lab Aquat Anim Nutr & Feed, Zhanjiang 524025, Peoples R China;[2]Aquat Anim Precis Nutr & High Efficiency Feed Engn, Zhanjiang, Guangdong, Peoples R China;[3]Minist Agr, Key Lab Aquat Livestock & Poultry Feed Sci & Techn, Zhanjiang 524025, Peoples R China;[4]Guangdong Evergreen Feed Ind Co Ltd, Zhanjiang 524088, Peoples R China;[5]Bioform Biotechnol Guangdong Co Ltd, Foshan 528200, Peoples R China

年份：2023

卷号：272

外文期刊名：MICROBIOLOGICAL RESEARCH

收录：SCI-EXPANDED(收录号：WOS:000999684700001)、、EI(收录号：20231814038694)、Scopus(收录号：2-s2.0-85153948858)、WOS

基金：Funding This work was supported by the National Science Foundation of China (No. 31772864) , Guangdong Province Enterprise Sci-tech Commissioner (GDKTP2020017800) , National Key Research and Development Program of China (2019YFD0900200) and Natural Sci- ence Foundation of Guangdong Province (2018A030313154 & 2020A1515011129) .

语种：英文

外文关键词：Clostridium butyricum; Litopenaeus vannamei; Whole genome sequence; Growth performance

外文摘要：In this study, a strain of Clostridium butyricum was isolated from the intestine of Litopenaeus vannamei with the method of anaerobic microbial isolation and culture. Next, the probiotic properties of LV1 were evaluated with susceptibility tests, tolerance tests, and whole genome sequencing in vivo and in vitro, followed by the analysis of the effect of LV1 on the growth performance, immune response, and disease resistance of Litopenaeus vannamei. According to the results, the 16 S rDNA sequence of LV1 was 100% homolofgous to the reference sequence of Clostridium butyricum. Moreover, LV1 was resistant to several antibiotics including amikacin, streptomycin, and gentamicin and highly tolerated artificial gastric and artificial intestinal fluids. The whole genome of LV1 was 4625,068 bp in size and included 4336 coding genes. Among these genes, GO, KEGG, and COG databases exhibited the highest number of genes annotated to metabolic pathway classes and 105 genes annotated as glycoside hydrolases. Meanwhile, 176 virulence genes were predicted. The use of diets supplemented with 1.2 x 109 CFU/kg of LV1 live cells significantly increased the weight gain and specific growth rates of Litopenaeus vannamei and the activity of serum superoxide dismutase, glutathione peroxidase, acid phosphatase, and alkaline phosphatase (P < 0.05). Meanwhile, the use of these diets markedly improved the relative expression of intes-tinal immunity-and growth-related genes. In conclusion, LV1 has excellent probiotic properties. Specifically, the addition of 1.2 x 109 CFU/kg of LV1 live cells to the diet improved the growth performance, immune response, and disease-resistance of Litopenaeus vannamei.