文献类型：期刊文献

英文题名：HMG20A from Nile tilapia (Oreochromis niloticus) involved in the immune response to bacterial infection

作者：Zhang, Zhiqiang[1];Niu, Jinzhong[1];Li, Qi[1];Huang, Yongxiong[1];Jiang, Baijian[1];Wu, Yiqin[1];Huang, Yu[1,2,3];Jian, Jichang[1,2,3]

机构：[1]Guangdong Ocean Univ, Guangdong Higher Educ Inst,Key Lab Control Dis Aq, Coll Fishery,Southern Marine Sci & Engn Guangdong, Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang, Peoples R China;[2]Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China;[3]Guangdong Prov Engn Res Ctr Aquat Anim Hlth Asses, Shenzhen, Peoples R China

年份：2021

卷号：119

起止页码：499

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000714681100005)、、WOS

基金：This work was supported by National Natural Science Foundation of China (No. U20A2065, 32073006, 32002426) , National Key R&D Pro-gram of China (No. 2018YFD0900501) , Fund of Southern Marine Sci-ence and Engineering Guangdong Laboratory (Zhanjiang) (No. ZJW-2019-06) .

语种：英文

外文关键词：HMG20A; RNA interference; Nile tilapia; Streptococcus agalactiae; Immune response

外文摘要：High-mobility group 20 A (HMG20A) has important biological functions, such as inhibiting the differentiation of red blood cells and nerve cells, promoting the proliferation and metastasis of cancer cells, and regulating inflammatory reaction. However, the role of HMG20A in the response to bacterial infection in the economic fish Nile tilapia (Oreochromis niloticus) remains unclear. In this study, a HMG20A homolog was successfully identified and characterized from Nile tilapia (On-HMG20A), and its expression model and biological effects on bacterial infection were analyzed. The open reading frame (ORF) of On-HMG20A was 876 bp in length, which encoded 291 amino acids and possessed a HMG domain (High mobility group domains) and coiled coil region. Results of the expression model showed that On-HMG20A was widely distributed in immune-related tissues of healthy tilapia and upregulated in a time-dependent manner after being challenged by Streptococcus agalactiae. Meanwhile, knocking down the expression of On-HMG20A can reduce the inflammatory response of tilapia and the degree of tissue damage caused by S. agalactiae. Moreover, knocking down the expression of On-HMG20A can reduce the bacterial load of tilapia tissues after being challenged by S. agalactiae and improve the survival rate. Collectively, these results showed that On-HMG20A may be related to the immune response of Nile tilapia against bacterial infection.