文献类型：期刊文献

英文题名：Simultaneous determination of six triterpenic acids in some Chinese medicinal herbs using ultrasound-assisted dispersive liquid-liquid microextraction and high-performance liquid chromatography with fluorescence detection

作者：Wu, Hongliang[1,4];Li, Guoliang[2];Liu, Shucheng[3];Liu, Di[1,4];Chen, Guang[2];Hu, Na[1,4];Suo, Yourui[1];You, Jinmao[1,2]

机构：[1]Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining, Peoples R China;[2]Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu, Peoples R China;[3]Guangdong Ocean Univ, Coll Food Sci & Technol, Guangdong Prov Key Lab Aquat Prod Proc & Safety, Zhanjiang, Peoples R China;[4]Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China

年份：2015

卷号：107

起止页码：98

外文期刊名：JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

收录：SCI-EXPANDED(收录号：WOS:000351116900014)、、Scopus(收录号：2-s2.0-84920576614)、WOS

基金：This work was supported by The National Natural Science Foundation of China (No. 31301595, No.21475074 and NO.21275089), Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety (GDPKLAPPS1401) and PhD research start-up funds of Qufu Normal University (bsqd 2012017).

语种：英文

外文关键词：Triterpenic acids; Pre-column derivatization; High-performance liquid chromatography; Ultrasound-assisted dispersive liquid-liquid microextraction; Response surface methodology; Traditional Chinese medicinal herbs

外文摘要：A novel analytical method was developed for simultaneous determination of six triterpenic acids using ultrasound-assisted dispersive liquid-liquid microextraction (UA-DLLME) follow by high-performance liquid chromatography (HPLC) with fluorescence detection. Six triterpenic acids (ursolic acid, oleanolic acid, betulinic acid, maslinic acid, betulonic acid and corosolic acid) were extracted by UA-DLLME using chloroform and acetone as the extraction and disperser solvents, respectively. After the extraction and nitrogen flushing, the extracts were rapidly derivatized with 2-(12,13-dihydro-7H-dibenzo[a,g]carbazol-7-yl)ethyl4-methylbenzenesulfonate. The main experimental parameters affecting extraction efficiency and derivatization yield were investigated and optimized by response surface methodology (RSM) combined with Box-Behnken design (BBD). The limits of detection (LODs) and the limits of quantification (LOQs) were in the range of 0.95-1.36 ng mL(-1) and 3.17-4.55 ng mL(-1), respectively. Under the optimum conditions, the method has been successfully applied for the analysis of triterpenic acids in six different traditional Chinese medicinal herbs. (C) 2014 Elsevier B.V. All rights reserved.