文献类型：期刊文献

英文题名：Label-free fluorescent aptasensor for chloramphenicol based on hybridization chain reaction amplification and G-quadruplex/N-methyl mesoporphyrin IX complexation

作者：Zheng, Wentao[1];Li, Yubin[2];Zhao, Liting[2];Li, Ciling[2];Wang, Lei[1]

机构：[1]Guangdong Med Univ, Zhanjiang Cent Hosp, Zhanjiang 524045, Peoples R China;[2]Guangdong Ocean Univ, Fac Chem & Environm Sci, Zhanjiang 524088, Peoples R China

年份：2022

卷号：12

期号：29

起止页码：18347

外文期刊名：RSC ADVANCES

收录：SCI-EXPANDED(收录号：WOS:000814225400001)、、EI(收录号：20222712318357)、Scopus(收录号：2-s2.0-85133255822)、WOS

基金：This work was supported by the foundation and applied foundation research Joint-Youth Fund project of Guangdong province, China (2019A1515110648), the project of Enhancing School with Innovation of Guangdong Ocean University (2020KZDZX1108), and Zhanjiang Science and Technology Development Special Fund Competitive Distribution Project (2021A05152).

语种：英文

外文摘要：The use of the broad-spectrum antibiotic chloramphenicol (CAP) in food is strictly regulated or banned in many countries. Herein, for the sensitive, rapid, and specific detection of CAP in milk, a label-free fluorescence strategy was established based on guanine (G)-quadruplex/N-methyl mesoporphyrin IX (NMM) complex formation and hybridization chain reaction (HCR) amplification. In this system, CAP can specifically bind to an aptamer (Apt) to release an Apt-C sequence from double-stranded DNA (Apt center dot Apt-C). Apt-C, can further hybridize with a functional hairpin DNA probe to release a primer sequence. The released primer sequence causes HCR and the formation of a nicked double-helix polymer, which contains G-quadruplex DNA. The recognition of G-quadruplex DNA by the NMM fluorochrome results in fluorescence enhancement. Consequently, CAP can be quantitatively detected by measuring the fluorescence intensity at 612 nm. The reliability of the aptasensor method was confirmed by comparison with an enzyme-linked immunosorbent assay. The proposed aptasensor was found to have a limit of detection of 0.8 pg mL(-1) for CAP. Moreover, when the aptasensor was applied to the detection of CAP in milk samples, the average recoveries were 99.8-108.3% with relative standard deviations of 4.5-5.2%. Thus, this CAP detection method, which is rapid with high sensitivity and selectivity, has considerable potential for a wide range of food analysis applications.