文献类型：期刊文献

中文题名：马氏珠母贝磺基转移酶PmCHST9基因的分子特征与表达分析

英文题名：Molecular Characterization and Expression Analysis of Pm CHST9 Gene from Sulfotransferase of Pinctada martensii

作者：郝瑞娟[1,2];郑哲[1,2];王庆恒[1,2];邓岳文[1,2];焦钰[1,2];杜晓东[1,2]

机构：[1]广东海洋大学水产学院;[2]广东省珍珠养殖与加工工程技术研究中心

年份：2015

卷号：0

期号：11

起止页码：2387

中文期刊名：基因组学与应用生物学

外文期刊名：Genomics and Applied Biology

收录：北大核心2014、CSCD2015_2016、北大核心、CSCD

基金：广东省海洋与渔业局科技专项(A201308A10);国家自然基金项目(31372526,31272635,41206141)共同资助

语种：中文

中文关键词：马氏珠母贝;磺基转移酶;分子特征;表达分析

外文关键词：Pinctada martensii,Sulfotranferase,Molecular characterization,Expression analysis

中文摘要：糖胺聚糖在抗病毒、消炎和抗氧化等免疫反应发挥重要作用。磺基转移酶(sulfotransferase)是糖胺聚糖生物合成的关键酶,Carbohydrate sulfotransferase 9(CHST9)可以将磺酸基从3'-磷酸腺苷酰-5'磷酸硫酸盐(PAPS)转移到半乳糖残基非还原末端的4位碳上。为研究CHST9在马氏珠母贝免疫调节中的作用,本研究克隆得到马氏珠母贝CHST9(Pinctada martensii CHST9,Pm CHST9)c DNA全长序列并检测其在不同组织中的表达模式。利用RACE技术,得出Pm CHST9序列全长1 388 bp,其中5'UTR为122 bp,3'UTR为192 bp,开放阅读框(ORF)为1 074 bp,编码357个氨基酸;Pm CHST9氨基酸序列分子量为42 889.2 Da,等电点为9.28,脂溶性系数为81.32,总平均亲水性为-0.493;氨基酸序列分析得出Pm CHST9具有典型的磺基转移酶-2结构域和一个跨膜结构域;多序列比对结果显示Pm CHST9与其它物种的CHST9同源性较低;荧光定量PCR检测结果表明Pm CHST9在鳃中表达量最高,其次是足和肝胰腺。综上所述,Pm CHST9可能参与马氏珠母贝的免疫调节作用,为进一步阐述Pm CHST9在马氏珠母贝中的免疫机制提供基础资料。

外文摘要：Glycosaminoglycans(GAGs) play important roles in immune response including antiviral reaction, antiphlogosis and oxidation resistance. Sulfotransferase is a key enzyme in the biosynthesis of GAGs. Carbohydrate sulfotransferase 9(CHST9) catalyzes the transfer of sulfate from 3'-phosphoadenosine-5'-phosphosulfate(PAPS)to carbon 4 of the N-acetylgalactosamine residues. To study the immune functions of CHST9 in Pinctada martensii(Pm CHST9), we cloned the full length of Pm CHST9 c DNA and detected its expression pattern in different tissues.Using rapid amplification of c DNA ends technology, the full length of Pm CHST9 c DNA was 1 388 bp, which obtained a 5' UTR of 122 bp, a 3' UTR of 192 bp and a 1 074 bp of the open reading frame(ORF) which encoded357 amino acids. The predicted molecular weight was 42 889.2 Da, the isoelectric point was 9.28, the aliphatic index was 81.32 and the grand average of hydropathicity(GRAVY) was-0.493. Amino acids sequence analysis showed that Pm CHST9 had a typical sulfotranferase-2 domain and a transmembrane domain. Multiple sequence alignments exhibited Pm CHST9 with low homology to other species CHST9. In addition, Pm CHST9 was most highly expressed in the gill, second highly expressed in foot and hepatopancreas using the real-time PCR analysis.Our results suggested that Pm CHST9 involved in the immune adjustment function and offered new materials for Pm CHST in the innate immunity of Pinctada martensii.