文献类型：期刊文献

中文题名：黄鳍金枪鱼头蛋白酶解液及分级组分对小鼠脾淋巴细胞增殖的影响

英文题名：Effect of Tuna Head Enzymatic Hydrolysate and Its Portions on Mouse Spleen Lymphocyte Proliferation

作者：杨萍[1];蔡本利[1];洪鹏志[1]

机构：[1]水产品深加工广东普通高校重点实验室广东海洋大学食品科技学院,广东湛江524088

年份：2010

卷号：22

期号：3

起止页码：498

中文期刊名：天然产物研究与开发

外文期刊名：Natural Product Research and Development

收录：CSTPCD、、CSCD2011_2012、CSCD

基金：广东省海洋与渔业局科技兴海项目<金枪鱼综合加工利用>

语种：中文

中文关键词：金枪鱼;酶解;小鼠;淋巴细胞

外文关键词：tuna; enzymatic hydrolysate; mouse ;lymphocyte

中文摘要：研究黄鳍金枪鱼头蛋白酶解液(EPTH)及截留相对分子量(MWCO)10、5、3和1 kD的超滤分级组分Ⅰ(>10 kD)、组分Ⅱ(10～5 kD)、组分Ⅲ(5～3 kD)、组分Ⅳ(3～1 kD)和组分Ⅴ(<1 kD)对小鼠脾淋巴细胞增殖的影响。采用无菌分离小鼠脾淋巴细胞,MTT法检测EPTH及各分级组分对有和没有刀豆蛋白A(ConA)、脂多糖(LPS)诱导下脾淋巴细胞增殖的影响,半微量凯氏定氮法、测定EPTH及各分级组分的氮含量。结果显示组分Ⅱ、组分Ⅴ单独作用能明显促进淋巴细胞增殖,并显著促进ConA、LPS诱导的淋巴细胞增殖;EPTH及其余3组分均没有显著的促进或抑制作用。组分Ⅱ、组分Ⅴ富集了促进小鼠脾淋巴细胞增殖的因子;组分Ⅱ、组分Ⅴ蛋白质含量分别占EPTH的38.9%和5.39%。

外文摘要：To investigate the effect of tuna heads enzymatic hydrolysate （EPTH） and its portions of ultrafiltration membrane with molecular weight cut-off （MWCO） of 10,5,3 and 1 kD on mouse spleen lymphocyte proliferation. The lymphocytes were prepared from spleen. The effect of EPTH and its portions on the proliferation of spleen lymphocyte in response to ConA and LPS was detected by MTT. The contents of nitrogen of EPTH and its portions were detected by micro-Kjeldadl. The portion Ⅱ （ 10-5 kD） and portionV （ 〈 1 kD） significantly stimulated the mouse spleen lymphocyte proliferation with and without conA and LPS, EPTH and portion Ⅰ （ 〉 10 kD）, Ⅲ（5-3 kD）, Ⅳ （3-1 kD）had no significant stimulated or inhibited effect. The portion Ⅱ and portion V were rich in. stimulating factures. The protein contents of portion Ⅱ and portion V were 38.9% ,5.39% of EPTH respectively.