文献类型：期刊文献

英文题名：Analysis of T-2 toxin removal factors in a Lactococcus fermentation system

作者：Zhou L.-H.; Wang Y.-L.; Qiu M.; Shi Q.; Sun L.-J.; Liao J.-M.; Xu D.-F.; Liu Y.; Fang Z.-J.; Gooneratne R.

机构：[1]College of Food Science and Technology, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, Guangdong Ocean University, Zhanjiang, 524088, China;[2]National Marine Products Quality Supervision and Inspection Center, Zhanjiang, 524000, China;[3]Centre for Food Research and Innovation, Department of Wine, Food and Molecular Biosciences, Lincoln University, Lincoln, Canterbury, 7647, New Zealand

年份：2017

卷号：80

期号：9

起止页码：1471

外文期刊名：Journal of Food Protection

收录：Scopus(收录号：2-s2.0-85027967329)

语种：英文

外文关键词：Decontamination; Lactic acid bacteria; T-2 toxin

外文摘要：The objective of this work was to determine the bacterial strains and factors that most efficiently degrade T-2 toxin in foods or animal feed. To determine the most efficient strain and optimal incubation times for degradation of T-2, the rate of T-2 removal by three lactic acid bacteria strains was quantified by liquid chromatography plus tandem mass spectrometry after incubation in de Man Rogosa Sharpe broth with 50 ng mL-1 T-2 at 37°C for 96 h. Various components of the most efficient degradation strain fermentation systems were extracted, and the ability to remove T-2 was assayed. Lactococcus lactis CAMT22361 was the most efficient degradation strain for removing T-2. Yeast extract powder interfered with L. lactis CAMT22361 in the degradation process. T-2 toxin was removed by various components of the L. lactis CAMT22361 cells in the following order: nonprotein material of the extracellular fraction > protein in the extracellular fraction > whole cell ≈ cell wall > cell intracellular matrix fluid. T-2 removal rates were 54.08% ± 0.79%, 43.65% ± 0.84%, 43.09% ± 0.87%, 41.98% ± 0.8%, and 23.45% ± 0.66%, respectively. The nonprotein fraction in the extracellular fluid was most likely the key component in L. lactis CAMT22361 and hence would be the most desirable cellular component to be used to remove T-2 from food or feed. Copyright ?, International Association for Food Protection.