文献类型：期刊文献

英文题名：Transcriptomic Landscape of Gut Microbiota-Host Interactions Reveals Domestication-Related Changes in the Pearl Oyster Pinctada maxima

作者：Huang, Jing[1,2];Zhang, Teng[2];Xie, Dongmei[1];Zheng, Zhe[1,2];Yang, Chuangye[2,3,4,5,6];Liao, Yongshan[3];Wang, Qingheng[2,3,5,6];Deng, Yuewen[1,2,3,5,6]

机构：[1]Guangdong Ocean Univ, Shenzhen Inst, Shenzhen 518120, Peoples R China;[2]Guangdong Ocean Univ, Fisheries Coll, Zhanjiang 524088, Peoples R China;[3]Guangdong Ocean Univ, Pearl Res Inst, Zhanjiang 524088, Peoples R China;[4]Guangdong Zunding Pearl Co Ltd, Zhanjiang Engn Res Ctr Marine Pearl Cultivat & Hig, Zhanjiang 524088, Peoples R China;[5]Pearl Breeding & Proc Engn Technol, Res Ctr Guangdong Prov, Zhanjiang 524088, Peoples R China;[6]Guangdong Prov Key Lab Aquat Anim Dis Control & Hl, Zhanjiang 524088, Peoples R China

年份：2026

卷号：16

期号：18

外文期刊名：ANIMALS

收录：SCI-EXPANDED(收录号：WOS:001891015100001)、、Scopus(收录号：2-s2.0-105051951884)、WOS

基金：This research was funded by Research on Breeding Technology of Candidate Species for Guangdong Modern Marine Ranching (Grant no. 2025-MRB-00-001), Department of Education of Guangdong Province (Grant no. 2024ZDZX4061), National Key Research and Development Program of China (Grant no. 2022YFD2401303), Youth S & T Talent Support Programme of Guangdong Provincial Association for Science and Technology (Grant no. SKXRC2025389), Shellfish & Algae Industry Innovation Team of Guangdong Modern Agricultural Technology System (Grant no. 2024CXTD23), and the earmarked fund for CARS-49.

语种：英文

外文关键词：Pinctada maxima; domesticated population; adaptation; transcriptome; gut microbiota

外文摘要：The offspring of domesticated Pinctada maxima exhibited various physiological and microbial adjustments to the complex and dynamic conditions of coastal environments. To support the restoration of P. maxima genetic resources and explore the molecular mechanisms underlying these phenotypic responses, we conducted a comparative analysis of the intestinal transcriptome and microbiota of wild parental and domesticated generations. Each sample generated an average of 43,600,525 clean reads, which were mapped to the P. maxima reference genome with mapping rates ranging from 63.91% to 79.48%, and a total of 3007 differentially expressed genes (DEGs) were subsequently identified. Gene Ontology analysis revealed that the DEGs were enriched in organic acid metabolism, and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that the DEGs were enriched in glycosphingolipid biosynthesis and xenobiotic metabolism via cytochrome P450. Microbiota profiling revealed significant compositional shifts at phylum and genus levels, with increased alpha diversity in F1; dominant phyla transitioned towards Spirochaetota and Bacteroidota, and functional predictions pointed to enhanced metabolic and immune capacities. Quantitative validated the up-regulation of immune genes (PmHR96h, PmIAP) and down-regulation of calcium-signaling genes (PmCaM, PmHSP90), consistent with RNA-seq data. Collectively, these coordinated transcriptomic and microbial alterations reflect a multifaceted host-microbiome adaptive response to nearshore conditions. Our findings provide valuable molecular markers and microbial indicators for selective breeding and health monitoring, offering a scientific basis for improving the resilience of P. maxima aquaculture under changing environmental conditions.