文献类型：期刊文献

英文题名：Transcriptome analysis of the pearl oyster (Pinctada fucata) hemocytes in response to Vibrio alginolyticus infection

作者：Wang, Zhongliang[1,2];Wang, Bei[1,3];Chen, Gang[1,2];Jian, Jichang[1,3];Lu, Yishan[1,3];Xu, Youhou[5];Wu, Zaohe[3,4]

机构：[1]Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524025, Peoples R China;[2]Guangdong Prov Key Lab Aquaculture South China Se, Zhanjiang 524025, Peoples R China;[3]Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang 524025, Peoples R China;[4]Zhongkai Univ Agr & Engn, Guangzhou 510225, Guangdong, Peoples R China;[5]Guangxi Key Lab Beibu Gulf Marine Biodiveis Conse, Qinzhou 535099, Peoples R China

年份：2016

卷号：575

期号：2

起止页码：421

外文期刊名：GENE

收录：SCI-EXPANDED(收录号：WOS:000366537600008)、、Scopus(收录号：2-s2.0-84942060966)、WOS

基金：This work was financially supported by grants from the National Natural Science Foundation of China (NSFC) (31202023), Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Qinzhou University (2015KB05) and Project of Enhancing School With Innovation (GDOU2015050214). We are especially grateful for the critical comments from the anonymous reviewers. The authors also thank Novogene Bioinformatics Technology (Beijing, China) for transcriptome sequencing, and also thank Genedenovo Biotechnology Co., Ltd. (Guangzhou, China) and Biomarker Technologies Co., Ltd. (Beijing, China) for assisting in the revision of this manuscript.

语种：英文

外文关键词：RNA sequencing; Immune response; Bacterial challenge; Hemocyte

外文摘要：The pearl oyster Pinctada fucata is cultured widely for production of marine pearls in China, while mass mortalities, likely related to pathogenic infections, have occurred frequently in juvenile, mother and operated oysters. To address this issue, understanding host defense mechanisms of P. fucata against pathogenic challenge is extremely important. In the present study, a comparative analysis of hemocyte transcriptomes of P. fucata before and after Vibrio alginolyticus infection was conducted using the Illumina/Hiseq-2000 RNA-Seq technology. A total of 56,345,139 clean reads were generated and then assembled into 74,007 unigenes with an average length of 680 bp and an N50 of 1197 bp. Unigenes were annotated by comparing against non-redundant protein sequence (nr), non-redundant nucleotide (nt), Swiss-Prot, Pfam, Gene Ontology database (GO), Clusters of Orthologous Groups (COG) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases, and 29,615 unigenes (40.01%) were annotated in at least one database. There were 636 genes (518 up-regulated and 118 down-regulated) that were significantly differentially expressed after bacterial challenge, and among which 369 were associated with 122 pathways, including classical immune-related pathways, such as 'MAPK signaling pathway', 'Chemokine signaling pathway', 'Apoptosis' and Wnt signaling pathway'. These findings provide information on the pearl oyster innate immunity and may contribute to developing strategies for management of diseases and long-term sustainability of P. fucata culture. (C) 2015 Elsevier B.V. All rights reserved.