文献类型：期刊文献

英文题名：Histone acetyltransferase GCN5-mediated lysine acetylation modulates salt stress aadaption of Trichoderma

作者：Li, Zhe[1,2];Zhang, Hao[1];Cai, Chunjing[1];Lin, Zhong[3];Zhen, Zhen[3];Chu, Jie[1];Guo, Kai[1]

机构：[1]Qilu Univ Technol, Biol Inst, Shandong Acad Sci, Jinan 250014, Peoples R China;[2]Chinese Acad Sci, State Key Lab Environm Chem & Ecotoxicol, Res Ctr Ecoenvironm Sci, Beijing 10085, Peoples R China;[3]Guangdong Ocean Univ, Fac Chem & Environm Sci, Zhanjiang 524088, Peoples R China

年份：2022

卷号：106

期号：8

起止页码：3033

外文期刊名：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000778055000002)、、EI(收录号：20221511937242)、Scopus(收录号：2-s2.0-85127580916)、WOS

基金：This research is supported by the National Natural Science Foundation of China (21607169; 41977125; 41907033; 21254356), the National Natural Science Foundation of Shandong Province (ZR202102270296), the Natural Science Foundation of Guangdong Province (2019A1515011948), the Youth Science Funds of Shandong Academy of Sciences (2020QN0019), the Program for Innovation Team in the University of Jinan (2019GXRC033), the Key R&D Program of Yantai (2019ZDCX016), and the Special Science and Technology Project of Rizhao (2019CXZX2205).

语种：英文

外文关键词：Trichoderma viride; Epigenetic modification; Histone acetylase GCN5; Salt tolerance; Sodium compartmentation; Antioxidant

外文摘要：Trichoderma viride has a wide range of applications in plant growth promotion, biological control, cellulase production, and biomass utilization. Salinity is a major limitation to Trichoderma strains in the natural environment and fermentation environment, and to improve the adaptability of Trichoderma to salt stress is of great significance to its applications in industry and agriculture. Histone acetylation plays important roles in the regulation of physiological and biochemical processes including various stress responses. GCN5 is the most representative histone acetylase, which plays vital roles in chromatin remodeling of promoters to facilitate the transcription activation. In this paper, we identified a GCN5-encoding gene TvGCN5 in T. viride Tv-1511, and characterized the function and regulating mechanism of TvGCN5-mediated acetylation of histone H3 in the salt adoption of Tv-1511, by constructions of the deletion mutants (Tv-1511-Delta GCN5) and overexpression mutants (Tv-1511-GCN5-OE) of TvGCN5. Results showed that compared with wild-type Tv-1511, the over-expression of TvGCN5 resulted in the longer mycelia diameter and more biomass under salt stress. Furthermore, Tv-1511-Delta GCN5 strains obtained the improved sodium (Na+) compartmentation and antioxidant capacity by upregulating the transcriptional levels of genes encoding PM H+ -ATPase, vacuolar H+ -ATPase, and antioxidant enzymes. Notably, the changes in the transcriptional expressions of these genes are tightly modulated by the TvGCN5-mediated acetylated level of histone H3 in their promoter regions. In all, these results reveal that TvGCN5 plays an important role in stress tolerance of T. viride Tv-1511, and provides potential insight to facilitate the application of epigenetic modulation in the expanding utilization of Trichoderma.