文献类型：期刊文献

英文题名：Unveiling the potentials of bacteriocin (Pediocin L50) from Pediococcus acidilactici with antagonist spectrum in a Caenorhabditis elegans model

作者：Chelliah, Ramachandran[1];Saravanakumar, Kandasamy[2];Daliri, Eric Banan-Mwine[1];Kim, Joong-Hark[2,3];Lee, Jung-Kun[2,3];Jo, Hyeon-yeong[1];Kim, Se-Hun[1];Ramakrishnan, Sudha Rani[4];Madar, Inamul Hasan[5];Wei, Shuai[2,6];Rubab, Momna[1];Barathikannan, Kaliyan[1];Ofosu, Fred Kwame[1];Subin, Hwang[1];Eun-ji, Park[1];Yeong, Jung Da[1];Elahi, Fazle[1];Wang, Myeong-Hyeon[2];Park, Jong Hwan[7];Ahn, Juhee[2];Kim, Dong-Hwan[8];Park, Sung Jin[1];Oh, Deog-Hwan[1]

机构：[1]Kangwon Natl Univ, Coll Agr & Life Sci, Dept Food Sci & Biotechnol, Chunchon 24341, Gangwon Do, South Korea;[2]Kangwon Natl Univ, Coll Biomed Sci, Dept Med Biotechnol, Chunchon 24341, Gangwon Do, South Korea;[3]Erom Co Ltd, Chunchon 24427, Gangwon Do, South Korea;[4]Kyungpook Natl Univ, Sch Food Sci & Biotechnol, Daegu, South Korea;[5]Bharathidasan Univ, Sch Life Sci, Dept Biochem, Thiruchirappalli, Tamil Nadu, India;[6]Guangdong Ocean Univ, Coll Food Sci & Technol, Guangdong Prov Key Lab Aquat Prod Proc & Safety, Zhanjiang 524088, Peoples R China;[7]Chonnam Natl Univ, Lab Anim Med, Gwangju, South Korea;[8]Kangwon Natl Univ, Kangwon Inst Inclus Technol, Chunchon 24341, Gangwon Do, South Korea

年份：2020

卷号：143

起止页码：555

外文期刊名：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

收录：SCI-EXPANDED(收录号：WOS:000515212500056)、、EI(收录号：20242616304797)、WOS

基金：This research was financially supported by the Ministry of SMEs and Startups (MSS), Korea, under the "Regional Specialized Industry Development Program(R&D, Project P00004989)" supervised by the Korea Institute for Advancement of Technology (KIAT). The authors would like to thank Hyun-Ah Lee at the Central laboratory of the Kangwon National University for instructing and technical support with a HPLC and a NMR and the Korean Basic Science Institute (Chuncheon), Bio-imaging center, Myeong-seon Jeong is also thanked for technical assistance with the EF-TEM analysis.

语种：英文

外文关键词：Probiotic; Pediococcus acidilatici; Antimicrobial peptide (pediocin); C-13 NMR; LCMS

外文摘要：Human-milk-based probiotics play a major role in the early colonization and protection of infants against gastrointestinal infection. We investigated potential probiotics in human milk. Among 41 Lactic acid bacteria (LAB) strains, four strains showed high antimicrobial activity against Escherichia coli 0157:H7, Listeria monocytogenes ATCC 15313, Bacillus cereus ATCC 14576, Staphylococcus aureus ATCC 19095, and Helicobacter pylori. The selected LAB strains were tested in simulated gastrointestinal conditions for their survival. Four LAB strains showed high resistance to pepsin (82%-99%), bile with pancreatine stability (96%-100%), and low pH (80%-94%). They showed moderate cell surface hydrophobicity (22%-46%), auto-aggregation abilities (12%-34%), and 70%-80% co-aggregation abilities against L. monocytogenes ATCC 15313, S. aureus ATCC 19095, B. cereus ATCC 14576, and E. coli 0157:H7. All four selected isolates were resistant to gentamicin, imipenem, novobiocin, tetracycline, clindamycin, meropenem, ampicillin, and penicillin. The results show that Pediococcus acidilatici is likely an efficient probiotic strain to produce < 3 Kda pediocin-based antimicrobial peptides, confirmed by applying amino acid sequences), using liquid chromatography mass spectrometry and HPLC with the corresponding sequences from class 2 bacteriocin, and based on the molecular docking, the mode of action of pediocin was determined on LipoX complex, further the C-13 nuclear magnetic resonance structural analysis, which confirmed the antimicrobial peptide as pediocin. (C) 2019 Elsevier B.V. All rights reserved.