文献类型：期刊文献

英文题名：Sucrose-nonfermenting 1 kinase activates histone acetylase GCN5 to promote cellulase production in Trichoderma

作者：Li, Zhe[1,2];Cai, Chunjing[1];Huo, Xuexue[1];Li, Xuan[1,2];Lin, Zhong[3]

机构：[1]Qilu Univ Technol, Biol Inst, Jinan 250014, Peoples R China;[2]Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 10085, Peoples R China;[3]Guangdong Ocean Univ, Fac Chem & Environm Sci, Zhanjiang 524088, Peoples R China

年份：2023

卷号：107

期号：15

起止页码：4917

外文期刊名：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

收录：SCI-EXPANDED(收录号：WOS:001008461900001)、、EI(收录号：20232514263526)、Scopus(收录号：2-s2.0-85161799487)、WOS

基金：This research is supported by the Natural Science Foundation of Shandong Province (ZR202102270296), the Education and Industry Integration Innovation Pilot Project of Qilu University of Technology (Shandong Academy of Sciences) (No. 2022JBZ01-06), the Program for Innovation Team in the University of Jinan (2019GXRC033), the National Natural Science Foundation of China (21607169; 41977125; 41907033), the Natural Science Foundation of Guangdong Province (2019A1515011948), the Foundation of International Technology Cooperation Project from Qilu University of Technology (Shandong Academy of Sciences) (2022GH026), and the Youth Science Funds of Shandong Academy of Sciences (2020QN0019).

语种：英文

外文关键词：Trichoderma viride; Cellulase production; SNF1 kinase; Histone acetylase GCN5; Epigenetic modification

外文摘要：Trichoderma serves as the primary producer of cellulases and hemicellulases in industrial settings as it readily secretes a variety of cellulolytic enzymes. The protein kinase SNF1 (sucrose-nonfermenting 1) can enable cells to adapt to changes in carbon metabolism by phosphorylating key rate-limiting enzymes involved in the maintenance of energy homeostasis and carbon metabolism within cells. Histone acetylation is an important epigenetic regulatory mechanism that influences physiological and biochemical processes. GCN5 is a representative histone acetylase involved in promoter chromatin remodeling and associated transcriptional activation. Here, the TvSNF1 and TvGCN5 genes were identified in Trichoderma viride Tv-1511, which exhibits promising activity with respect to its ability to produce cellulolytic enzymes for biological transformation. The SNF1-mediated activation of the histone acetyltransferase GCN5 was herein found to promote cellulase production in T. viride Tv-1511 via facilitating changes in histone acetylation. These results demonstrated that cellulolytic enzyme activity and the expression of genes encoding cellulases and transcriptional activators were clearly enhanced in T. viride Tv-1511 mutants in which TvSNF1 and TvGCN5 were overexpressed, with concomitant changes in histone H3 acetylation levels associated with these genes. GCN5 was also found to be directly recruited to promoter regions to alter histone acetylation, while SNF1 functioned upstream as a transcriptional activator that promotes GCN5 upregulation at the mRNA and protein levels in the context of cellulase induction in T. viride Tv-1511. These findings underscore the important role that this SNF1-GCN5 cascade plays in regulating cellulase production in T. viride Tv-1511 by promoting altered histone acetylation, offering a theoretical basis for the optimization of T. viride in the context of industrial cellulolytic enzyme production.