文献类型：期刊文献

中文题名：免疫鸡群新城疫病毒的分离与分子鉴定

英文题名：Isolation and Molecular Identification of Newcasttle Disease Virus from Immunized Chicken Flocks

作者：刘铀[1];刘艳芬[2];陈绍红[1];谢为天[2];马龙[3]

机构：[1]湛江海洋大学生化中心,广东湛江524088;[2]湛江海洋大学农学院,广东湛江524088;[3]湛江市畜牧局,广东湛江524038

年份：2006

卷号：26

期号：6

起止页码：597

中文期刊名：中国兽医学报

外文期刊名：Chinese Journal of Veterinary Science

收录：CSTPCD、、北大核心2004、CSCD2011_2012、北大核心、CSCD

语种：中文

中文关键词：新城疫病毒(NDV);分子鉴定;RT—PCR

外文关键词：Newcasttle disease virus （NDV）;molecular identification ; RT-PCR

中文摘要：从免疫鸡群中分离到9株新城疫病毒（NDV），其中5株为强毒株，4株为中强毒株。用RT—PCR扩增F基因cDNA片段，并将其分别克隆到pGEM—Teasy载体中。序列分析结果表明，上述分离株F基因长度为1662bp，编码553个氨基酸，其F蛋白的氨基酸序列同源性为96．0％～99．8％；但与常见疫苗株的氨基酸同源性仅为87.6％～92.2％，F蛋白裂解位点序列为^112R-R-Q／R—K—R—FI—G^119,均属于基因Ⅶ型NDV。

外文摘要：Nine strains of Newcasttle disease virus（NDV） were isolated from immunized chicken flocks, 5 isolates of which were velogenic and the others were mesogenic. The cDNA fragments of F gene of 9 NDV strains above were amplified by RTPCR. The amplified fragments were cloned into pGEM-Teasy vector and the recombinant plasmids were sequenced. The results showed that the F gene from all of the NDV isolates consisted of 1 662 bp ,coding for 553 amino acids. The amino acid sequence homology among the 9 NDV isolates came to 96.0%-99.8% ,but the amino acid sequence homology between the 9 isolates and other NDV vaccine strains（LaSota,B1,F48E9 and Roakin） were relatively lower,from 87.6% to 92.2%. The sequence of cleavege site of F protein were ^112R-R-Q/R-K-R-F-I-G^119,all of the 9 isolates belonged to the genetype Ⅶ.