文献类型：会议论文

英文题名：Optimization of the SPME-GC-MS techniques for analyzing headspace volatiles in shrimp head of P. Borealis

作者：Xie, Wan Cui[1]; Lian, Xin[1]; Yang, Xi Hong[1]; Yang, Ya Dong[1]; Mao, Dan Hui[1]

机构：[1] Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China

会议论文集：Advances in Environmental Technologies

会议日期：April 19, 2013 - April 21, 2013

会议地点：Guilin, China

语种：英文

外文关键词：Volatile organic compounds - Flavor compounds - Gas chromatography - Shellfish - Mass spectrometry - Sulfur compounds - Extraction

外文摘要：To investigate the headspace volatiles in shrimp head P. borealis, optimized conditions were evaluated for solid-phase microextraction coupled to gas chromatography-mass spectrometry method. SPME fibers of 100-μm polydimetylsiloxane, 65-μm polydimetylsiloxane-divinylbenzene, 75-μm carboxen-polydimetylsiloxane, and 50/30-μm divinylbenzene-carboxen-polydimetylsiloxane (DVB/CAR/PDMS) were compared. The influence of several parameters such as type of fibers, volume of sample, extraction time, extraction temperature, and state of samples was systematically investigated. Suitable reproducibility and sensitivity were achieved by combining DVB/CAR/PDMS of 50/30-μm thickness as the liquid phase of SPME, 1.5 g of shrimp head lyophilized powder, 40 °C of isolation temperature and 40 min of isolation time. A total of 62 volatile compounds have been confirm, including hydrocarbons, ketones, alcohols, aldehydes, esters, nitrogen-containing compounds and sulfur-containing compounds. 3,5-octadien-2-one (E,Z), 3,5-octadien-2-one (E,E), 1-penten-3-one, 6-octen-2-one, ethyl acetate and alcohols such as 1-penten-3-ol, 2-penten-1-ol and 1-octen-3-ol were predicted as key compounds for SH flavor. ? (2013) Trans Tech Publications, Switzerland.