文献类型：期刊文献

英文题名：Heat Stress-Induced Dysbiosis of Porcine Colon Microbiota Plays a Role in Intestinal Damage: A Fecal Microbiota Profile

作者：Hu, Canying[1,2];Patil, Yadnyavalkya[3];Gong, Dongliang[1];Yu, Tianyue[1];Li, Junyu[4];Wu, Lianyun[1];Liu, Xiaoxi[1];Yu, Zhichao[1];Ma, Xinbing[1];Yong, Yanhong[4];Chen, Jinjun[1];Gooneratne, Ravi[3];Ju, Xianghong[1,2,4]

机构：[1]Guangdong Ocean Univ, Agr Coll, Dept Anim Sci, Zhanjiang, Peoples R China;[2]Guangdong Ocean Univ, Shenzhen Inst, Shenzhen, Peoples R China;[3]Lincoln Univ, Fac Agr & Life Sci, Dept Wine Food & Mol Biosci, Lincoln, New Zealand;[4]Guangdong Ocean Univ, Coll Agr, Dept Vet Med, Zhanjiang, Peoples R China

年份：2022

卷号：9

外文期刊名：FRONTIERS IN VETERINARY SCIENCE

收录：SCI-EXPANDED(收录号：WOS:000773679300001)、、Scopus(收录号：2-s2.0-85127187345)、WOS

基金：Funding This study was supported by the National Natural Science Foundation of China [grant numbers 31472243, 31902314]; Natural Science Foundation of Guangdong Province, China [grant number: 2019A1515011142]; the Project of Enhancing School with Innovation of Guangdong Ocean University [grant number: GDOU230419057]; the Basic Research Project of Shenzhen Science and Technology Innovation Commission (JCYJ20190813142005766).

语种：英文

外文关键词：heat stress; colonic microbiome; inflammation in the colon; pigs; mice

外文摘要：The pathological mechanisms of gastrointestinal disorders, including inflammatory bowel disease (IBD), in pigs are poorly understood. We report the induction of intestinal inflammation in heat-stressed (HS) pigs, fecal microbiota transplantation from pigs to mice, and explain the role of microorganisms in IBD. 24 adult pigs were subjected to HS (34 +/- 1 degrees C; 75-85% relative humidity for 24h) while 24 control pigs (CP) were kept at 25 +/- 3 degrees C and the same humidity. Pigs were sacrificed on days 1, 7, 14, 21. Colonic content microbiome analyses were conducted. Pseudo-germ-free mice were fed by gavage with fecal microbiota from HS-pigs and CP to induce pig-like responses in mice. From 7 d, HS-pigs exhibited fever and diarrhea, and significantly lower colonic mucosal thickness, crypt depth/width, and goblet cell number. Compared with each control group, the concentration of cortisol in the peripheral blood of HS pigs gradually increased, significantly so on days 7, 14, and 21 (P < 0.01). While the concentration of LPS in HS pigs' peripheral blood was significantly higher on days 7, 14 (P < 0.01), and 21 (P < 0.05) compared with that of the control group. The colonic microbiome composition of HS-pigs was different to that of CP. By day 14, opportunistic pathogens (e.g., Campylobacterales) had increased in HS-pigs. The composition of the colonic microbiome in mice administered feces from HS-pigs was different from those receiving CP feces. Bacteroides were significantly diminished, Akkermansia were significantly increased, and intestinal damage and goblet cell numbers were higher in mice that received HS-pig feces. Moreover, we verified the relevance of differences in the microbiota of the colon among treatments. Heat stress promotes changes in gut microbiome composition, which can affect the colonic microbial structure of mice through fecal microbiota transplantation; the molecular mechanisms require further investigation. This study enhanced our understanding of stress-induced inflammation in the colon and the increase in diarrhea in mammals subjected to prolonged HS. Our results provide useful information for preventing or ameliorating deficits in pig production caused by prolonged exposure to high temperatures.