文献类型：期刊文献

英文题名：Characterization of LPS-induced TNFα factor (LITAF) from orange-spotted grouper, Epinephelus coioides

作者：Cai, Jia[1,2];Huang, Youhua[1];Wei, Shina[1];Ouyang, Zhengliang[1];Huang, Xiaohong[1];Qin, Qiwei[1]

机构：[1]Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China;[2]Guangdong Ocean Univ, Coll Fishery, Zhanjiang 524025, Peoples R China

年份：2013

卷号：35

期号：6

起止页码：1858

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000330158800019)、、WOS

基金：This work was supported by grants from the National Basic Research Program of China (973) (2012CB114402, 2012CB114404), the National Natural Science Foundation of China (31172445, 31172437, 30930070) and the National High Technology Development Program of China (863) (2012AA092201).

语种：英文

外文关键词：Epinephelus coioides; LPS-induced TNF alpha factor (LITAF); Singapore grouper iridovirus (SGIV); Apoptosis; Viral replication

外文摘要：Lipopolysaccharide-induced TNF alpha factor (LITAF) is an important transcription factor that mediates cell apoptosis and inflammatory response. In the present study, we cloned and characterized a LITAF gene from orange-spotted grouper (Epinephelus coioides) (Ec-LITAF). Ec-LITAF encoded a predicted 142 amino acid protein which shared 74% identity to sablefish (Anoplopoma fimbria) LITAF homolog. Multiple amino acid alignment showed that Ec-LITAF contained a typical LITAF domain with two CXXC motifs. Phylogenetic analysis indicated that Ec-LITAF was closely related to that of sablefish. Ec-LITAF mRNA was widely expressed in different tissues and its expression level in spleen was up-regulated after Singapore grouper iridovirus (SGIV) infection. Subcellular localization analysis revealed that the distribution of Ec-LITAF showed diffuse and aggregated patterns in cytoplasm. Interestingly, the distribution of Ec-LITAF overlayed with a viral LITAF homolog (vLITAF) encoded by SGIV. Overexpression of Ec-LITAF in vitro up-regulated the expression of tumor necrosis factors (TNF1 and TNF2) and TNF receptors (TNFR1 and TNFR2), and the expression of itself initiated apoptosis in fish cells. In addition, overexpression of Ec-LITAF not only accelerated SGIV infection induced CPE and cell death, but also increased viral gene transcription. Taken together, our data suggested that Ec-LITAF might play crucial roles during SGIV replication. (C) 2013 Elsevier Ltd. All rights reserved.